CVB3对不同营养状态的HeLa细胞mTOR信号通路调控的影响  

Regulation of mTOR signal pathway in HeLa cells under different nutritional conditions by Coxsackie virus B3

作　　者：成亮[1] 陈淳媛[1] 杨作成[1] 朱咏贵[1] 黄利华[2] 闾宏伟[2] 

机构地区：[1]中南大学湘雅三医院儿科,长沙410013 [2]中南大学湘雅三医院医学实验中心,长沙410013

出　　处：《中南大学学报（医学版）》2013年第1期20-25,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(30973233)~~

摘　　要：目的:探讨不同营养状况对柯萨奇病毒B3(CVB3)感染HeLa细胞后mTOR信号通路调控的影响。方法:HeLa细胞的培养方法:非饥饿法用常规含10%胎牛血清细胞培养液换液培养24 h后次日再用CVB3干预;饥饿法用不含胎牛血清的培养基换液培养24 h后次日再干预。将HeLa细胞分为非饥饿法病毒组与对照组、饥饿法病毒组与对照组4组,采用RT-PCR检测不同时间点HeLa细胞CVB3外壳蛋白、mTOR和p70S6K mRNA表达。结果:非饥饿法:病毒组mTOR,p70S6K mRNA表达与对照组比较仅在12,24 h,差异有统计学意义(P<0.05),而饥饿法病毒组各时间点均低于对照组(均P<0.05);饥饿法病毒组和对照组HeLa细胞mTOR表达均高于非饥饿法(均P<0.05),而p70S6K mRNA差异无统计学意义(均P>0.05)。结论:CVB3使HeLa细胞mTOR-p70S6K信号通路表达下调,饥饿法mTOR表达高于非饥饿法。Objective： To explore the changes of mTOR signal pathway in HeLa cells under different nutritional conditions infected with Coxsackie virus B3 （CVB3）. Methods： The HeLa cells were cultured with two methods： the conventional culture method cultured HeLa cells with medium with 10% fetal bovine serum for 24 h and changed the medium next day, and then infected with CVB3; the serum starvation method cultured HeLa cells with medium without fetal bovine serum for 24 h, and then infected with CVB3. The expression of the coat protein of CVB3j mTOR, p70S6K mRNA was detected with RT-PCR at different time points. Results： The virus group showed the expressions of mTOR and p70S6K mRNA were significantly higher than those in the control group at 12 h and 24 h （P〈0.05） in the conventional culture. The virus group showed the expressions of mTOR and p70S6K mRNA were lower than those in the control group （all P〈0.05） in the starvation serum. The expression of mTOR mRNA in the starvation serum virus group was higher than that in the conventional culture virus group （all P〈0.05） and the control group. The expression ofp70S6K mRNA was not significantly different in the two groups （P〉0.05）. Conclusion： CVB3 can down-regulate the expressions of mTOR and p70S6K mRNA. The mTOR expression in the starvation serum is higher than that in the conventional culture.

关 键 词：柯萨奇病毒B3 HELA细胞 营养剥夺 MTOR 

分 类 号：R725.4[医药卫生—儿科]