转化生长因子β1(TGF-β1)对胃癌细胞microRNA表达谱的影响  

The effect of TGF-β 1 on microRNA profiling of gastric cancer cells

作　　者：周海燕[1] 王宽松[1] 胡忠良[1] 何琼琼[1] 李勇[2] 文继舫[1] 

机构地区：[1]中南大学基础医学院病理学系,长沙410013 [2]湖南省儿童医院普外科,长沙410007

出　　处：《复旦学报（医学版）》2013年第1期15-20,共6页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(81001080;30901452);教育部博士点新教师基金项目(20070533099)~~

摘　　要：目的探讨转化生长因子β1(transforming growth factorβ1,TGF-β1)对胃癌细胞BGC823 microRNA(miRNA)表达谱的影响及其意义。方法应用microRNA芯片检测TGF-β1处理胃癌细胞BGC823前后的miRNA表达谱;对miRNA表达谱进行差异性分析;实时荧光定量RT-PCR验证差异表达的miRNA;用生物信息学技术分析差异表达miRNA可能调控的靶基因及其意义。结果 TGF-β1处理胃癌细胞BGC823 24 h和36 h后,miRNA的表达谱存在6个相同的差异表达miRNA,包括3个(miR-27a,miR-29b-1和miR-194)表达上调,3个(miR-193b,miR-574-3p和miR-130b)表达下调。实时荧光定量RT-PCR结果与芯片一致。结论 TGF-β1能够影响胃癌细胞miRNAs的表达,这些上调或下调表达的miRNAs可能参与了胃癌的浸润转移。Objective To explore the effect and significance of TGF-13 1 on microRNA profiling of gastric cancer cells. Methods microRNA array was used to detect microRNA profiling of BGC823 cells after treated with TGF-13 1. Real-time RT-PCR was used to validate differentially expressed microRNAs. Bioinformatics analysis was used to predict target genes of differentially expressed microRNA and its significance. ResuLts There were six differential microRNAs after treated by TGF-13 1 for both 24 and 36 hours of BGC823. Three （miR-27a,miR-29b-1 and miR-194） of them were up-regulated,others （miR-193b,miR-574-3p and miR-131）h） were down-regulated. The results of real- time RT-PCR were consistent with the chips. Conclusions TGF-beta 1 can affect miRNAs profiling of gastric cancer cells. These miRNAs which up-regulated or down-regulated may he involved in invasion and metastasis of gastric cancer.

关 键 词：microRNA芯片 转化生长因子β1(TGF-β1) 肿瘤浸润与转移 

分 类 号：R735.2[医药卫生—肿瘤]