Midkine(MDK)在肝细胞癌(HCC)中的表达及其临床意义  

Expressions and clinical significance of Midkine(MDK) in human hepatocellular carcinoma(HCC)

作　　者：朱文伟[1] 张巨波[2] 郭磊[2] 张博[2] 叶青海[2] 

机构地区：[1]复旦大学附属肿瘤医院胰腺肝胆外科,上海200032 [2]复旦大学附属中山医院肝癌研究所肝外科,上海200032

出　　处：《复旦学报（医学版）》2013年第1期55-59,共5页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金项目(80171993)~~

摘　　要：目的探讨Midkine(MDK)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达以及检测血清MDK对于肝癌诊断的初步临床意义。方法通过免疫组织化学染色和Western blot法检测50例临床样本(包含肝癌,肝硬化及正常肝组织)及7种不同肝癌细胞系中MDK表达情况;进一步通过酶联免疫吸附反应定量检测120例不同受试人群的血清样本,分析血清MDK在诊断肝癌中的初步临床意义。结果肝癌组织中MDK表达阳性率显著高于肝硬化(77%vs.30%,P<0.01)及正常肝组织(77%vs.0%,P<0.001);Western blot检测结果显示,MDK在多株肝癌细胞系中表达上调;此外,HCC患者血清MDK的中位数水平(1.195 ng/mL,0.84～1.71)较正常人(0.102 ng/mL,0.02～0.53;P<0.01)、HBV相关肝硬化(0.57 ng/mL,0.26～0.67;P<0.05)和HCV相关肝硬化(0.34 ng/mL,0.09～0.56;P<0.01)患者显著升高。结论 MDK在HCC患者中的表达显著上调,血清检测MDK可作为临床诊断肝癌的重要方法。Objective To investigate the expression level of Midkine （MDK） in hepatoeellular carcinoma （HCC） and evaluate the clinical diagnostic value of serum MDK for HCC. Methods MDK expression was separately assessed by immunohistochemistry and Western blot in 50 tissue samples （30 from HCC tissues, 10） from cirrhotic tissues and 10 from normal liver tissues） and 7 different cell lines. Serum MDK levels were detected by Enzyme-linked immunosorbent assay in 120 participants including HCCs and controls. Its diagnostic value of HCC was further analyzed. Results MDK level was significantly elevated in HCC tissues compared with cirrhotic tissues （77% vs. 30% P〈0.01） and normal liver tissues （77% vs. 0%; P〈0. 001）. In addition, MDK was widely up-regulated in HCC cell lines. Moreover, serum MDK was significantly elevated in H（＇C patients （1. 195 ng/mI.,0.84- 1.71 ） compared with healthy donors （I）. 102 ng/rnl.,0.02 - 0.53; P〈 0.01 ）, HBV related cirrhosis （0. 57 ng/mL, 0. 26 - 0.67 PM0.05） and HCV related cirrhosis （0.34 ng/mL. 09 - 0. 56;P〈0. 01）. Conclusions MDK is significantly elevated in HCC patients. Serum MI）K may serve as a novel diagnostic tumor marker for HCC.

关 键 词：肝细胞癌(HCC) Midkine(MDK) 肿瘤标记物 

分 类 号：R730.23[医药卫生—肿瘤]