视黄酸对人脐血淋巴细胞生长因子基因的调节  

REGULATION OF THE GROWTH FACTORS GENES BY RETINOIC ACID IN HUMAN CORD BLOOD LYMPHOCYTES

作　　者：杨毅[1] 王卫平[1] 苏贻新[1] 

机构地区：[1]上海医科大学儿科医院,上海200032

出　　处：《营养学报》2000年第2期124-126,共3页Acta Nutrimenta Sinica

基　　金：国家自然科学基金! (39470 6 13)

摘　　要：目的　探讨维生素 A促进免疫细胞功能的机制。方法　采用逆转录——聚合酶链反应 ,检测分析视黄酸 (RA)对体外培养的脐血淋巴细胞 (CBL)胰岛素样生长因子 1 (IGF- )及两类受体 (IGF- R,- R)基因表达水平的调节。结果　在加入生理浓度 RA培养 6～ 2 4 h CBLIGF- ,IGF- R和 IGF- R基因表达显著上调。结论　在 RA对免疫细胞的作用中 IGFs的调节可能是一条重要途径。Objective:[WT5BZ] To explore the mechanism of immune promotion by retinoic acid (RA). [WT5FZ]Methods:[WT5BZ] RT PCR (reverse transcription polymers chain reaction) was used to detect the genes expression of insulin like growth factor I (IGF Ⅰ) and IGF receptors (IGF ⅠR and IGF ⅡR) and regulation by RA in human cord blood lymphocytes (CBL). [WT5FZ]Results:[WT5BZ] The results showed that RA at physiological serum level significantly upregulated the levels of IGF Ⅰ, IGF ⅠR and IGF ⅡR mRNA in human cord blood lymphocytes after 6 to 24 hours culture. [WT5FZ]Conclusion:[WT5BZ] It is suggested that the enhancement of IGFs gene expression is an important way for vitamin A to regulate immune cells functions.[WT5”FZ]

关 键 词：视黄酸 人脐血淋巴细胞 IGF-1 基因调节 

分 类 号：R151[医药卫生—营养与食品卫生学]