茶多酚对急性百草枯中毒大鼠治疗作用的实验研究  

Experimental study on the therapeutic effect of tea polyphenols against acute paraquat poisoning in rats

作　　者：刘丽娜[1] 赵金垣[2] 陈莉[2] 刘和亮 丁丽华[2] 

机构地区：[1]重庆医科大学第二临床学院急诊科,400010 [2]北京医科大学第三医院职业病研究中心,100083

出　　处：《中华劳动卫生职业病杂志》2000年第3期158-160,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：美国中华医学基金资助项目

摘　　要：目的　探讨茶多酚 (teapolyphenols ,TP)对急性百草枯中毒的治疗作用。方法　大鼠用百草枯(paraquat,PQ)灌胃 (2 5 0mg/kg)染毒后 3h再经口灌注TP ,分别测定染毒后 8、2 4、48、72h大鼠血浆和支气管 肺泡灌洗液 (BALF)中丙二醛 (MDA)含量及超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)的活力 ,并观察肺组织结构改变。结果　PQ染毒后血浆及BALF中MDA含量显著高于对照组 ,差异有显著性 (P <0 .0 1) ;血浆SOD活力下降 ,差异有显著性 (P <0 .0 1) ,48h后GSH Px稍升高 ;BALF中SOD、GSH Px均下降 ,但前者 48h后升高 ,差异有显著性 (P <0 .0 1)。给予TP治疗后 ,与中毒组比较 ,血浆和BALF中MDA含量下降 ,差异有显著性 (P <0 .0 5 ) ;GSH Px和SOD活力逐渐升高 ,差异有显著性 (P <0 .0 5 )。肺组织病理学检查可见PQ中毒后肺泡壁充血、增厚 ,炎症细胞浸润 ,并有局灶性出血 ;给予TP后 ,肺泡壁充血、出血均有减轻。Objective To study the therapeutic effect of tea polyphenols(TP) against acute poisoning by paraquat(PQ). Methods SD rats were given(p.o.) TP at 3 h after oral administration of PQ(250 mg/kg).The level of MDA,superoxide dismutase(SOD) and glutathione peroxidase(GSH Px) both in plasma and bronchoalveolar lavage fluid(BALF) were detected at 8,24,48,72 h respectively after TP treatment. Results Compared with control group, the level of MDA in PQ poisoning group increased significantly both in plasma and BALF( P <0.01);SOD in plasma was decreased( P <0.01),GSH Px showed some increase 48 h after poisoning.The activities of SOD and GSH Px in BALF were all decreased,but SOD showed some increase 48 h later.Compare TP treated group with PQ poisoning group,the MDA level decreased both in plasma and BALF( P <0.05),the SOD and GSH Px activities were increased as well( P < 0.05). The pathological changes in lungs caused by PQ were mainly shown as congestion,leukocyte infiltration,thickness and local hemorrhage in alveolar wall.After TP treatment,congestion and hemorrhage of alveoli got some improvement. Conclusion Administration of TP could improve the lipid peroxidation damage caused by acute poisoning of PQ.

关 键 词：急性百草枯中毒 药物疗法 茶多酚 实验研究 

分 类 号：R595.4[医药卫生—内科学]