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作 者:张金花[1,2] 贺莉芳[1] 杨霞霞[1] 万启惠[1]
机构地区:[1]遵义医学院寄生虫学教研室,贵州遵义563003 [2]川北医学院附属医院检验科,四川南充637000
出 处:《国际检验医学杂志》2012年第16期1941-1942,共2页International Journal of Laboratory Medicine
摘 要:目的研究家蝇胚胎细胞抗菌肽对人髓样白血病细胞K562的抑制作用。方法利用脂多糖诱导家蝇胚胎细胞MDEC-07114,提取抗菌肽,将家蝇胚胎细胞抗菌肽分为40、80、160、320和640μg/mL5个实验组,PBS作为阴性对照组,采用MTT法测定不同浓度抗菌肽对人髓样白血病细胞K562和人脐静脉血管内皮细胞的抑制作用并绘制K562细胞生长曲线。结果家蝇胚胎细胞抗菌肽对肿瘤细胞K562具有明显抑制作用,其浓度与抑制率具有显著相关性,浓度越高,作用时间越长,对K562的抑制杀伤越强。各浓度组对人体正常细胞均无抑制作用。结论家蝇胚胎细胞抗菌肽可抑制肿瘤细胞生长而不杀伤人体正常细胞。Objective To research the inhibitory action of the antibacterial peptide from the theembryonic cells of the musca do- mestica on the human myeloid leukemia K562.Methods The logarithmic growth phase′s embryonic cells of the musca domestica were induced by LPS.The antibacterial peptide was extracted from supernatant fluid.The antibacterial peptide was prepared 40 , 80 , 160 , 320and 640 μ g / mL five density groups by PBS.The MTT experiments were made for human myeloid leukemia cells K562and the human umbilical vein vascular endothelial cells.The growth curves of K562cells in the above five density groups were made by MTT method and the tumour cells of isometric culture medium were been the control groups.Results The antibacterial peptide from the theembryonic cells of the musca domestica had remarkable inhibition activities on K562cells.There was a correlation betwa the inhibition rates and antibacterial peptide concentrations.Along with time extension and medicine density increasing , K562 cell mortality rate rised.Conclusion The antibacterical peptide from the embryonic cells of the musca domestica has significance in- hibitory activity on human myeloid leukemia cells K562 , and has no inhibition activity on human umbilical vein vascular endothelial cells.
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