hsa-miR-100的生物信息学分析  

Bioinformatics analysis of hsa-miR-100

作　　者：付海龙[1,2] 徐广峰[2,3] 史春梅[3,4] 季晨博[3,4] 郭锡熔[3,4] 赵亚萍[1,2] 

机构地区：[1]蚌埠医学院,安徽蚌埠233030 [2]中国人民解放军第82医院检验科,江苏淮安223001 [3]南京医科大学儿科研究所,江苏南京210029 [4]南京医科大学附属南京妇幼保健院儿童保健科,江苏南京210004

出　　处：《国际检验医学杂志》2012年第18期2177-2180,共4页International Journal of Laboratory Medicine

基　　金：江苏省自然科学基金资助项目(BK2012666);江苏省淮安市产学研合作促进计划专项资金资助项目(HAC201026)

摘　　要：目的对hsa-miR-100进行靶基因、功能预测等生物信息学分析,为深入研究hsa-miR-100功能提供理论和试验基础。方法利用Pubmed检索miRNA-100相关文章,通过miRBase、NCBI、UCSC Browser等在线工具分析hsa-miR-100序列,应用miRanda、TargetScan及PicTar预测hsa-miR-100靶基因,结合已证实的靶基因进行功能富集分析和信号通路富集分析。结果 hsa-miR-100与多种肿瘤发生、发展有关,其序列在各物种间具有高度保守性。hsa-miR-100靶基因功能富集于基因沉默、染色质沉默、细胞生物合成负性调节等(P<0.01),涉及肿瘤信号通路、溶酶体信号通路、凋亡信号通路等信号转导通路(P<0.05)。结论 hsa-miR-100可能参与肿瘤发生相关的生物学过程。Objective To predict targets and functions of hsa-miR-100 by bioinformatics analysis,so as to lay foundations and provide theoretical basis for the further studies of hsa-miR-100 biological function. Methods Current studies of miR-10O were re- trieved by Pubmed. The sequence of hsa-miR-100 was got from miRBase database,NCBI,UCSC Browser. Target genes of hsa-miR- 100 were predicted by miRanda,TargetScan and PieTar,and then analyzed by gene ontology and pathway enrichment analysis. Resuits Current studies showed that hsa-miR-100 was related with multiple tumorigenesis, miR-100 was highly conserved among dif- ferent species. Gene ontology analysis demonstrated the target genes of hsa-miR-100 were enriched in gene silencing,chromatin si- lencing,negative regulation of biosynthetic process and other biological processes （P〈0.01）. The pathway analysis indicated the target genes mainly involved in cancer signaling,lysosome signaling,apoptosis signaling and other pathways （P〈0.05）. Conclusion The target genes of hsa-miR-100 enriched in multiple biological processes were relevant to the tumor signaling pathways.

关 键 词：微RNAS hsa-miR-100 生物信息学 靶基因 肿瘤 

分 类 号：R730.2[医药卫生—肿瘤]