HBsAg和HBsAb双阳患者前C/C区基因突变分析  

Analysis of precore/core gene mutations in hepatitis B virus carriers with coexistence of HBsAg and HBsAb

作　　者：王蕾[1] 宁小晓[1] 王珊[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]苏州大学,江苏苏州215021

出　　处：《国际检验医学杂志》2012年第18期2200-2201,2203,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨乙型肝炎表面抗原(HBsAg)和乙型肝炎表面抗体(HBsAb)同时阳性(以下简称双阳)患者前C/C区基因突变的特点及其与S区基因突变的关系。方法选取18例双阳患者,对前C/C区及S区基因序列扩增并测序,分析测序结果。结果 18例双阳患者中,检出前C/C区氨基酸突变者7例;前C/C区发生突变与未发生突变者比较,其S区氨基酸的突变次数及突变率明显增加(P<0.05);7例前C/C区氨基酸突变患者中,4例为前C区nt1896突变,其中1例为HBeAg(-),3例为HBeAg(+)。结论双阳患者不仅S区氨基酸突变增多,其前C/C区氨基酸突变也明显增加;双阳患者前C区nt1896突变更常见于HBeAg阳性者,可能与双阳患者病毒株的复杂性有关。Objective To analyze the characteristics of precore/core gene mutations in hepatitis B virus carriers with coexistence of HBsAg and HBsAb（double positivity）. Methods Specimens collected from 18 cases with double positivity were detected for the mutation of precore/core gene and S gene. Results 7 cases with precore/core gene mutations were demonstrated,who were with more S gene mutations and higher mutation rate than those cases without precore/core mutation（P〈0.05）. 4 of the cases with pre core/core gene mutations were positive with nt1896 mutation in precore gene,of which 1 case was HBeAg-negative and 3 cases HBeAg-positive. Conclusion Hepatitis B virus carriers with double positivity might be not only with more S gene mutations, bul also with more precore/core gene mutations, nt1896 mutation in hepatitis B virus carriers with double positivity could be more corn mon in HBeAg-positive patients than HBeAg-negative patients,which might be associated with the complexity of HBV isolates.

关 键 词：肝炎病毒 乙型 肝炎表面抗原 乙肝 肝炎表面抗体 乙肝 前C C区 基因突变 

分 类 号：R512.62[医药卫生—内科学]