双孢蘑菇As2796全长cDNA文库的构建及鉴定  

Construction and Identification of Full-length cDNA Library of Agaricus bisporus As2796

作　　者：陈美元[1] 

机构地区：[1]福建省农业科学院食用菌研究所,福建福州350014

出　　处：《福建农业学报》2012年第11期1201-1204,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省财政专项--福建省农科院科技创新团队建设项目(2012)

摘　　要：双孢蘑菇As2796是我国的主栽品种。为方便获得相关的目的基因,构建了该菌株的全长cDNA文库,文库初始库容达3.3×106 cfu.mL-1,插入片段长度范围0.7～4.0kb,平均长度约1.5kb,重组率约82%,全长率约35%。文库扩增出了5个目的基因全长编码序列(CDS),表明该文库的有效性,今后可用于扩增所需的双孢蘑菇基因的全长cDNA或CDS。A.bisporus As2796 is the main cultivar in China.For easily cloning the related target genes,the full-length cDNA library of As2796 was constructed.The library had a content of 3.3×106 cfu·mL-1,an insertion range of about 0.7-4.0 kb,an average cDNA length of 1.5 kb,a recombination efficience of about 82%,and full-length ratio of about 35%.The coding sequences（CDS） of 5 target genes were got from the library by PCR amplification,which proved the validity of the library for amplifying full-length cDNA or CDS of A.bisporus genes in need.

关 键 词：食用菌 基因文库 编码序列 

分 类 号：S828[农业科学—畜牧学]