吉林地区正常汉族人群载脂蛋白E基因多态性分布状态的研究  

DISTRIBUTION OF APOLIPOPROTEIN E GENE POLYMORPHISM IN NORMAL HAN NATIONALITYPOPULATION OF JILIN DISTRICT

作　　者：刘华欣[1] 曹丽春[1] 罗正里[1] 黄晶波[1] 任旷[1] 王承志 王吉伟[2] 

机构地区：[1]第四军医大学吉林军医学院,132013 [2]湖南医科大学分子生物学研究中心,410018

出　　处：《中国现代医学杂志》2000年第7期23-24,共2页China Journal of Modern Medicine

摘　　要：研究载脂蛋白 (Apo)E基因多态性在吉林地区正常汉族人群中的分布状态。方法 :应用聚合酶链反应———限制性片段长度多态性 (PCR -RFLP)方法先扩增出ApoE基因第 4外显子内的 2 2 7bp片段 ,继以限制性切酶Cfol酶解消化 ,8%聚丙烯酰胺凝胶电泳 ,最后银染显带判定基因型 ,并计算等位基因频率。结果 :本实验共检测分析了 2 0 6例吉林地区正常汉族人的ApoE基因型及等位基因频率。结果表明纯合子ε3 /3 基因型频率及ε3 等位基因频率最高。ε2 高于ε4。且ε2 随年龄增长有增加趋势。结论 :该方法快速、准确 ,分辨率高。ApoE基因多态性分布的研究为ApoE生理功能的研究及进一步临床应用打下基础。Objective: To study the distribution of Apolipoprotein E gene polymorphism in normal Han nationality population of Jilin district. Methods: The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technigue was used to amplify 227 base-pair DNA fragments in the fourth exon of Apo E gene and to digest them with restriction endonuclease CfoI. Then, electrophoresis on 8% polyacrylamid gel for the cleavaged products was carried out. Subsequently their genotypes were determined by silver staining and allele frequencies were calculated. Results: In 206 healthy individuals of Han ethnicgroup, the frequencies of homozygous genotype ε3/3 andε3 allele were the highest, ε2 allele frequency was higher than that ofε4 andε2 allele frequency tends to increase with the group-ages. Conclusions: This method is rapid and precise, showing high discriminating rate. Study on the distribution of Apolipoprotein E gene polymorphism would play an important role for the research of ApoE physiological function and further clinical application.

关 键 词：APOE基因 基因多态性 基因型 等位基因 

分 类 号：Q343[生物学—遗传学]