阿尔茨海默病患者血浆3-NT浓度的变化及其临床意义  

The significance of determination of plasm 3-NT levels in patients with Alzheimer disease

作　　者：殷晓菁[1] 胡玲玲[1] 彭誉[2] 周华[2] 邱晨红[2] 孙沁怡[2] 王锋[2] 谢红娜[2] 赵中[2] 

机构地区：[1]无锡市第三人民医院神经内科,江苏无锡214041 [2]南京医科大学附属苏州医院神经内科,江苏苏州215001

出　　处：《苏州大学学报（医学版）》2012年第6期837-840,852,共5页Suzhou University Journal of Medical Science

基　　金：南京医科大学科技发展基金面上资助项目(2010NJMU107);苏州市科技发展计划(社会发展;农业科技)项目(SYSD2011110)

摘　　要：目的探讨血浆3-硝基酪氨酸(3-NT)浓度与阿尔茨海默病(AD)患者认知功能损害的关系。方法应用简易精神状态检查量表(MMSE)检测入组患者的认知功能,将其分为3组,即正常对照组(NC,37例)、血管性痴呆组(VD组,41例)、AD组(48例),分别用酶联免疫吸附试验(ELSIA)测定3组患者的血浆3-NT浓度,并对AD组患者的血浆3-NT浓度与MMSE得分及各个认知领域分值进行相关性分析。结果 NC组、VD组、AD组患者血浆3-NT浓度分别为(55.09±9.63)nmol/l、(70.42±11.45)nmol/l、(119.46±21.82)nmol/l,AD组患者血浆3-NT浓度明显高于NC组、VD组(均P<0.05);AD组患者血浆3-NT浓度与MMSE得分成明显负相关(r=-0.638,P<0.05);AD组患者血浆3-NT浓度与记忆力、定向力、注意力和计算能力均成明显负相关(均P<0.05)。结论血浆3-NT浓度测定有可能作为判断AD患者认知功能损害的生化指标,亦可作为AD与VD鉴别诊断的生物学指标。Objective To explore the relationship between plasm 3-nitrotyrosine （3-NT） levels and Alzheimer disease （AD）. Methods The subjects were 48 patients with AD and 78 controls ,including 41 patients with vascular dementia （VD） and the 37 normal controls （NC）. Cognitive function and plasm 3-NT levels in three groups were tested by Mini mental state examination （MMSE） and enzyme linked immunosorbent assay （ ELISA）, respectively. Results The levels of plasm 3-NT were （ 119.46 ± 21.82 ） nmol/LinAD patients, （70. 42 ± 11.45） nmol/L in VD, and （55. 09 ±9. 63） nmol/L in NC, respectively （ all P 〈 0.05 ）. The correlation analysis revealed that the plasm 3-NT levels were negatively correlated with the total MMSE scores （r = -0. 638 ,P 〈 0.05）. Multivariable linear regression showed that the plasm 3-NT levels were also negatively correlated with the memory scores,the orientation scores , the attention scores and the calculation in the AD groups （ all P 〈 0.05 ）. Conclusion 3-NT may be used as the biochemical indicator for observing AD, and may be used for differentiating AD and VD.

关 键 词：阿尔茨海默病 血管性痴呆 3-硝基酪氨酸 简易精神状态检查量表 

分 类 号：R749.16[医药卫生—神经病学与精神病学]