原发性肝癌组织中IL-18BPa和IL-18BPc的差异表达  

The different expressions of IL-18 BPa and IL-18 BPc in human primary hepatic carcinoma

作　　者：陈家洁[1] 吴建成[1] 陈祖涛[1] 查翔远[2] 李燕[3] 刘永萍[1] 

机构地区：[1]苏州大学附属第一医院感染病科,江苏苏州215006 [2]铜陵市第一人民医院,安徽铜陵244000 [3]黄石市第一医院,湖北黄石435001

出　　处：《苏州大学学报（医学版）》2012年第6期845-848,共4页Suzhou University Journal of Medical Science

基　　金：苏州市国际科技合作计划项目(SWH0925)

摘　　要：目的检测原发性肝癌组织及癌周组织IL-18BPa/IL-18BPc的表达,初步探讨IL-18BPa/IL-18BPc在原发性肝癌中的分布及异同点。方法应用免疫组织化学染色法(SP法)分别检测肝癌患者癌组织、癌周组织各34例(均为男性)、肝囊肿患者的正常肝脏组织5例中IL-18BPa/IL-18BPc的表达。结果 IL-18BPa亚型在肝癌的癌周组织、癌组织阳性表达率分别为94.11%和34.7%,而正常肝组织几乎不表达。癌周组织与其他两组间的差异有高度统计学意义(均P<0.01);而IL-18BPc亚型在肝癌的癌周组织、癌组织阳性表达率分别为38.24%和85.29%,同样在正常肝组织几乎不表达。肝癌组织与其他两组间的差异有统计学意义(均P<0.01)。结论 IL-18BPa亚型在肝癌的癌周组织中高表达可能与促进肿瘤局部新生血管的形成有关,而肝癌组织中IL-18BPc亚型高表达在原发性肝癌的发生和发展过程中起着重要作用,提示不同亚型的IL-18BP的功能不完全一致。Objective To study the expression of interleukin-18 binding protein a （IL-18BPa） and interleukin-18 binding protein c（IL-18BPc） in tumorou tissue and peri-tumorou tissue,and to explore the similarities and differences of the distribution of IL-18BPa and IL-18BPc in human primary hepatic carci- noma. Methods The expression of IL-18BPa and IL-18BPc was detected in the specimens of 34 cases of primary hepatic carcinoma（PHC） and 5 healthy hepatocellular tissues by immunohistochemical staining. Results The positive expression rate of IL-18BPa in tumorous tissue and peri-tumorous tissue was 34.7% and 94.11% ,but IL-18BPa was seldom expressed in normal liver tissue. IL-18BPa was overex- pressed in peri-tumorous tissues of primary hepatic carcinoma, that was significantly different compared with that in the other 2 groups（all P 〈0.01 ）. The positive expression rate of IL-18BPe in tumorou tissue and peri-tumorou tissue was 85.29% and 38.24% , and IL-18BPc was also seldom expressed in normal liver tissue. IL-18BPc was over expressed in tumorou tissues of primary hepatic carcinoma, that was signifi- cantly different compared with that in the other 2 groups （ all P 〈 0.01 ）. Conclusion The over expres- sion of IL-18BPa may be related to promote local angiogenesis in PHC. IL-18BPc somewhat plays a role in the occurrence and development process of PHC. So different subtypes of interleukin-18 binding protein have different roles and functions.

关 键 词：白细胞介素18结合蛋白亚型 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]