东亚飞蝗消化系统蛋白质组双向电泳的分析  

The Differential Analysis of Two-Dimensional Gel Electrophoresis for the Alimentary System from Locusta Migratoria Manilensis (Meyen)

作　　者：李燕良[1] 邬玉兰[1] 闫浩[1] 杨芷菁[1] 陈义坤[1] 刘志刚[1] 

机构地区：[1]深圳大学过敏反应与免疫学研究所,广东深圳518060

出　　处：《江西师范大学学报（自然科学版）》2012年第6期569-573,共5页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家"863"计划(2006AA100308);国家自然科学基金(30871752);深圳大学校创新科研团队基金(200904);深圳市重点实验室组建基金(SW201110010)资助项目

摘　　要：为了优化东亚飞蝗消化系统双向电泳技术,建立东亚飞蝗消化系统蛋白表达图谱,探讨雌、雄东亚飞蝗消化系统蛋白组分的差异,利用pH值为3~10和pH值为4~7的胶条分别对雌、雄东亚飞蝗消化系统进行双向电泳分析,进行蛋白组学分析.研究结果发现：雌性东亚飞蝗消化系统蛋白种类多于雄性,雌性东亚飞蝗消化系统特异蛋白中偏酸性蛋白数量与偏碱性蛋白相当,而雄性东亚飞蝗消化系统特异蛋白中酸性蛋白多于碱性蛋白.在雌、雄东亚飞蝗消化系统相匹配蛋白中,含量差异达3倍以上的蛋白约占总匹配蛋白的50%.可见,雌、雄东亚飞蝗消化系统蛋白组分存在差异.Two-dimensional electrophoresis system was optimized and carried out to establish protein expression map of alimentary system from Locusta migratoria manilensis（Meyen） and then the proteomics difference was investigateed between female Locusta migratoria manilensis（Meyen） and male ones.Two-dimensional electrophoresis was carried in pH 4~7 and pH 3~10 in order to obtain the best condition and to analyze the proteomics difference between female Locusta migratoria manilensis（Meyen） and male ones.It was found that there were more kinds of protein in alimentary system from female Locusta migratoria manilensis than that in male ones.Among the special protein from female Locusta migratoria manilensis,the quantity of acid protein has the similar level with that of alkaline protein while quantity of acid protein is larger than that of alkaline protein in male Locusta migratoria manilensis.In addition,the quantity of the matched protein spots whose abundance in female or male is over 3fold compared with that in male or female is nearly 50% of the total matched protein.Therefore,protein components are different between the alimentary system of male and that of female.

关 键 词：东亚飞蝗 消化系统 蛋白组分 双向电泳 差异性分析 

分 类 号：Q78[生物学—分子生物学]