肿瘤坏死因子在胆囊癌及结石组织中的表达  

EXPRESSION OF TUMOR NECROSIS FACTOR IN GALLSTONE AND GALLBLADDER CARCINOMA TISSUE

作　　者：周连锁[1] 石景森[2] 王作仁[2] 王林[2] 

机构地区：[1]汕头大学医学院附属肿瘤医院腹外科,广东汕头515031 [2]西安医科大学第一附属医院肝胆外科,陕西西安710061

出　　处：《癌变．畸变．突变》2000年第3期139-142,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家卫生部科学技术基金资助项目! ( 96-1 -2 4 9)

摘　　要：目的 :研究从胆囊粘膜典型增生到胆囊癌的发展过程中肿瘤坏死因子 (TNF)的表达 ,进一步探讨它们在胆囊癌发生、发展中的意义。方法 :用原位杂交及免疫组织化学方法检测胆囊结石引起的粘膜典型增生、非典型增生及胆囊癌的TNFmR NA、TNF蛋白的表达。结果 :①TNFmRNA在胆囊粘膜典型增生、非典型增生及胆囊癌组织上皮细胞或癌细胞和单个核细胞(MNC)的阳性表达率及表达细胞数目均逐级增高。②在胆囊癌组织中 ,癌细胞及MNC表达TNFmRNA的细胞表达数目随肿瘤进展而增多 (P <0 .0 1 )。③TNF蛋白与TNFmRNA表达区域相似 ,阳性表达率较高。④癌细胞和MNC的TNFmRNA及蛋白表达均呈正直线相关。结论 :TNF参与了胆囊结石致癌的发病过程 ,并与胆囊癌的进展有关。Purpose: To study the expression of TNF-α mRNA and TNF-α protein in the development from hyperplasia, dysplasia to carcinoma of gallbladder mucosa and to discuss the pathogenesis of gallbladder mucosa and to discuss the pathogenesis of gallbladder carcinoma. Methods: In situ hybridization and immunohistochemistry were used to detect the expression of TNF-α mRNA, TNF-α protein in mucosa of hyperplasia, dysplasia and carcinoma of gallbladder. Results: ①The percentages of positive cells and the numbers of positive cells/per high power field (HPF) which expressed TNF-α mRNA in hyperplasia, dysplasia and carcinoma of gallbladder mucosa cell and mononuclear cells(MNC) were increasing. ②The numbers of carcinoma cells and MNC /HPF which expressed TNF-α mRNA increased with increasing tumor grades and size. ③The pattern of TNF-α protein expression was similar to that of TNF-α mRNA, but more frequent and widespread. ④There was a positive linear correlation between tumor cells and MNC of TNF-α mRNA and TNF-α expression. Conclusion: TNF-α was involved in the pathogenesis of gallbladder carcinoma induced by gallstone and the TNF-α expression in cancer cells may possibly be a marker for tumor staging.

关 键 词：胆囊结石 胆囊癌 免疫组化 原位杂交 TNF 

分 类 号：R735.803[医药卫生—肿瘤]