荧光共振能量转移增敏法测定锌  

Determination of Zinc by Fluorescence Resonance Energy Transfer Sensitization Method

作　　者：陈志兵[1,2] 查珺[2] 胡松[2] 陈辉德[2] 王克文[2] 刘信[2] 

机构地区：[1]自旋电子与纳米材料安徽省重点实验室培育基地宿州学院,安徽省宿州市汴河路71号234000 [2]宿州学院化学与生命科学学院,安徽省宿州市汴河路71号234000

出　　处：《光谱实验室》2013年第1期23-26,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(20871089);安徽省教育厅重点项目(KJ2012A267);宿州学院科研平台开放课题(2012YKF01);宿州学院大学生科研项目(KYLXLKYB11-15)

摘　　要：以巯基乙酸为稳定剂合成了CdTe量子点,并利用其与牛血清白蛋白(BSA)组成了荧光共振能量转移体系,考察BSA(供体)和CdTe量子点(受体)之间的荧光共振能量转移的最佳条件,建立了荧光共振能量转移增敏法测定Zn^(2+)的新方法。在pH 7.4的Tris-HCl缓冲溶液中,Zn^(2+)能对能量转移体系中CdTe量子点增敏从而测定锌的含量。Zn^(2+)浓度在0.49—4.88μg/mL浓度范围内与CdTe量子点荧光强度呈现良好线性关系(r=0.9996),检出限为0.13μg/mL,RSD为3.2%,平均回收率为99.5%(n=5)。方法适用于果汁饮料中微量锌的测定。CdTe quantum dots（QDs） were synthesized with thioglycolic acid（TGA ） as stabilizer.Fluorescence resonance energy transfer system was constituted by CdTe QDs and bovine serum albumin（BSA）,the optimal conditions of fluorescence resonance energy transfer between BSA （donor） and CdTe QDs（receptor） were investigated,and a new method for determination of zinc ions by fluorescence resonance energy transfer sensitization method was established.In Tris-HCl buffer solution at pH 7.4,zinc ions can enhance the fluorescence intensity of CdTe QDs in fluorescence resonance energy transfer system,thereby concentration of zinc ions was detected.Results showed that a linear relationship could be established between the fluorescence of CdTe QDs and the concentration of Zn^（2＋） in the range of 0.49—4.88μg/mL（r=0.9996）,the detection limit was 0.13μg/mL,RSD and the average recovery were 3.2%and 99.5%（n=5）,respectively.This method can be applied to the determination of trace zinc in fruit drinks.

关 键 词：CDTE量子点 牛血清白蛋白 荧光增敏 锌 

分 类 号：O657.32[理学—分析化学]