外源性TRAIL基因转染对胶质瘤C6细胞凋亡的实验研究  

Effect of exogenous TRAIL gene transfection on apoptosis of C6 glioma cells

作　　者：张相华[1] 涂汉军[1] 张力[1] 胡钧涛[1] 王晓勋[1] 

机构地区：[1]湖北医药学院附属太和医院神经外科一病区,湖北十堰442000

出　　处：《中华神经外科疾病研究杂志》2012年第6期512-515,共4页Chinese Journal of Neurosurgical Disease Research

基　　金：湖北省科技厅基金资助项目(2005AA301C28);湖北省卫生厅2005青年科技人才基金资助项目(QJX2005-15);湖北医药学院优秀中青年科技创新团队基金资助项目(2005CXX02)

摘　　要：目的探讨外源性肿瘤坏死因子相关凋亡诱导配体(TRAIL)基因对大鼠C6胶质瘤细胞的凋亡作用。方法 C6细胞在杜伯改良的Eagle培养基(DMEM),37℃,5%CO2条件下培养;质粒提取与转染;用Hoechst试剂盒检测C6细胞凋亡。结果转染重组质粒PDC315-TRAIL的C6细胞组凋亡率明显高于未转染的C6细胞组及转染空载体PDC315的C6细胞组(P<0.05)。结论外源性TRAIL基因可引起C6细胞凋亡。Objective To investigate the effects of exogenous tumor necrosis factor related apoptosis inducing ligand （TRAIL） gene on the apoptosis of C6 glioma cells. Methods The cells were cultured in 5% CO2 Dulbeccos modified Eagles minimum essential medium （DMEM） at 37℃.The plasmids were reconstructed and transfected. The apoptosis of C6 glioma cells was detected in Hoechst kits. Results Compared with C6 cells and CA-PDC315 cells, the apoptosis rate of C6-TRAIL cells was higher （P 〈 0. 05）. Conclusion Exogenous TRAIL gene can induce the apoptosis of C6 glioma cells.

关 键 词：C6胶质瘤细胞 TRAIL基因转染 细胞凋亡 

分 类 号：R739.41[医药卫生—肿瘤]