乳腺癌患者外周血中SBEM mRNA的表达及意义  

Expression and significance of SBEM mRNA in peripheral blood of patients with breast cancer

作　　者：陈忠[1] 陈聆[1] 孟凡祥[1] 杜鸿[2] 

机构地区：[1]江苏省苏州永鼎医院检验科,江苏苏州215200 [2]苏州大学附属第二医院检验科,江苏苏州215004

出　　处：《国际检验医学杂志》2012年第19期2327-2328,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨乳腺癌患者外周血SBEM mRNA表达情况及其与临床病理因素的相关性。方法运用巢式逆转录-聚合酶链反应(nRT-PCR)检测56例乳腺癌患者外周血SBEM mRNA表达情况,以乳腺良性肿瘤患者及健康者各20例作为对照。结果乳腺癌患者外周血SBEM mRNA表达阳性率为46.4%(26/56),乳腺良性肿瘤患者及健康者表达阳性率均为0.0%(0/20,P<0.05);乳腺癌患者外周血SBEM mRNA表达阳性率与患者有无淋巴结转移及TNM分期相关(P<0.05),但与年龄、肿瘤大小无关(P>0.05)。结论乳腺癌患者外周血SBEM mRNA可作为乳腺癌微转移的判断指标,对乳腺癌临床分期、预后判断和治疗起重要作用。Objective To explore the expression of SBEM mRNA in the peripheral blood from patients with breast cancer and its correlations with clinicopathological parameters.Methods Nested reverse transcription-polymerase chain reaction（nRT-PCR） was performed to investigate the expression status of SBEM mRNA in the peripheral blood from 60 cases of breast cancer,20 cases of benign breast diseases and 20 healthy volunteers.Results The positive rate of SBEM mRNA expression in breast cancer group was 46.4%（26/56）,whereas those in the other two groups were both 0.0%（P0.05）.The positive rate of SBEM mRNA expression was correlated with lymph node metastasis and TNM stage,but was not associated with age and tumor size.Conclusion The positive expression of SBEM mRNA in the peripheral blood of patients with breast cancer might serve as a diagnostic marker of micro-metastasis and be helpful for the correct clinical staging,prognostic diagnosis and treatment for the patients.

关 键 词：乳腺肿瘤 黏蛋白类 巢式逆转录聚合酶链反应 微转移 

分 类 号：R737.9[医药卫生—肿瘤]