急性白血病形态学与免疫分型分析  

Analysis of morphology and immunophenotyping of acute leukemia

作　　者：周萍[1] 唐吉斌[1] 章文[1] 

机构地区：[1]安徽省铜陵市人民医院临床检验中心,安徽铜陵244009

出　　处：《国际检验医学杂志》2012年第19期2331-2332,2334,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨形态学结合免疫分型对急性白血病(AL)的诊断价值。方法对78例AL患者骨髓标本进行基于染色技术的形态学和基于流式细胞术免疫分型检测,比较不同分型结果。结果形态学与免疫分型符合率为89.74%(70/78);分化程度较低、形态不典型及抗原系列交叉表达的AL形态学相对不易分型。急性髓细胞系白血病主要表达cMPO(96.72%)、CD13(78.68%)、CD117(70.49%)、CD33(67.21%);急性淋巴细胞系白血病主要表达CD19(82.35%)、cCD79a(70.58%)、CD7(17.64%)、cCD3(17.64%)。结论 AL诊断应结合形态学及免疫分型,二者不相符时,应进行染色体和融合基因检测;免疫分型有助于B、T细胞系及形态学难以分型的特殊类型AL的诊断。Objective To investigate the diagnostic value of morphology combined with immune genotyping for diagnosis of acute leukemia（AL）.Methods Morphological classification,based on various stain methods,and immunophenotyping,based on flow cytometry,were performed for the classification of 78 patents with AL.Results The consistence rate between the two methods was 89.74%（70/78）.Acute myeloid leukemia mainly expressed cMPO（96.72%）,CD13（78.68%）,CD117（70.49%） and CD33（67.21%）,while acute lymphocytic leukemia commonly expressed CD19（82.35%）,cCD79a（70.58%） and CD7（17.64%）.Conclusion The diagnosis of AL should be based on morphology,combined with immunophenotyping.For those with different diagnostic results for the two methods,chromosome and fusion gene should be further investigated.Immunophenotyping might contribute to identify B or T lymphoid lineages and to diagnose special types of AL with difficulties for morphology classification.

关 键 词：急性白血病 形态学 免疫分型 

分 类 号：R733.71[医药卫生—肿瘤]