健康妇女及宫颈上皮癌瘤患者HPV感染基因型分布特征研究  

A comparative study of HPV infective genotypes distribution in cells of normal cervix and tissues of cervical lesions

作　　者：龙秀荣 王志蕙[2] 耿建祥[2] 朴正爱[2] 肖蔚[2] 万美珍[2] 王旭波[2] 兰建云[2] 张劲松[2] 

机构地区：[1]南京市六合区人民医院病理科,江苏南京211500 [2]南京中医药大学附属第三医院病理科HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2012年第24期2958-2959,2962,共3页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项资助项目(2009-92)

摘　　要：目的探讨南京地区一般女性人群宫颈细胞、宫颈上皮内瘤变及宫颈癌组织中23种人乳头瘤病毒感染基因型别的分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1000例一般女性人群宫颈细胞、108例宫颈上皮内瘤变及62例宫颈癌组织标本进行23种HPV基因型别的检测,并对受检者进行相关资料分析。结果 1000例一般女性人群检出HPV感染者106例,总的HPV感染率为10.60%(106/1000);108例宫颈上皮内瘤变患者检出HPV感染者99例,总的HPV感染率为91.67%(99/108);62例宫颈癌患者检出HPV感染者52例,总的HPV感染率为83.87%(52/62)。结论基因扩增结合基因芯片检测技术可应用于宫颈细胞和组织标本,一次可检测23种HPV基因型别,特异性强,敏感性高,对中国女性宫颈HPV感染分子流行病学的调查、宫颈癌和癌前病变的防治及其疫苗的研究具有重要的意义。Objective To compare the clinical distribution of 23 kinds of human papillomavirus genotypes in cells of normal cervix and tissues of cervical lesions and its clinical significance.Methods Polymerase chain reaction and gene-chips technology were utilized for the detection of 23 kinds of HPV genotypes in cells from 1000 cases of normal cervix 、tissue specimens from 108 cases of cervical intraepithelial neoplasia（CIN） and from 62 cases of cervical cancers.And related materials of all subjects were analyzed.Results In 1 000 cases of normal cervix,total infection rate of HPV was 10.60%（106/1000）;In 108 cases of CIN,total infection rate of HPV was 91.67%（99/108）;In 62 cases of cervical cancers,total infection rate of HPV was 83.87%（52/62）.Conclusion Gene chip technology can detect single and multiple HPV genotypes in cervical cell and tissue samples with high sensitivity and specificity.Detection of HPV genotypes could be used to understand the prevalence situation of HPV infection and further to supply reference for the research and development of HPV vaccine fit for different regions.

关 键 词：细胞 组织 子宫颈 乳头状瘤病毒科 基因型 遗传学技术 

分 类 号：R737.33[医药卫生—肿瘤]