Umbilical cord-derived mesenchymal stem cells retain immunomodulatory and anti-oxidative activities after neural induction  

作　　者：Jianjun Li Dong Li Xiuli Ju Qing Shi Dakun Wang Fengcai Wei 

机构地区：[1]Department of Anesthesiology, Qilu Hospital, Shandong University [2]Cryomedicine Laboratory, Qilu Hospital, Shandong University [3]Department of Stomatology, Qilu Hospital, Shandong University

出　　处：《Neural Regeneration Research》2012年第34期2663-2672,共10页中国神经再生研究（英文版）

基　　金：supported by grants from the Shandong Province Science and Technology Program, GrantNo.2011GSF11801;the Innovation Fund Project of Shandong University, Grant No.2012ZD023;the Major StateBasic Research Development Program, Grant No.2012CB966504

摘　　要：The immunomodulatory and anti-oxidative activities of differentiated mesenchymal stem cells contribute to their therapeutic efficacy in cell-replacement therapy. Mesenchymal stem cells were isolated from human umbilical cord and induced to differentiate with basic fibroblast growth factor, nerve growth factor, epidermal growth factor, brain-derived neurotrophic factor and forskolin. The mesenchymal stem cells became rounded with long processes and expressed the neural markers, Tujl, neurofilament 200, microtubule-associated protein-2 and neuron-specific enolase. Nestin expression was significantly reduced after neural induction. The expression of immunoregulatory and anti-oxidative genes was largely unchanged prior to and after neural induction in mesenchymal stem cells. There was no significant difference in the effects of control and induced mesenchymal stem cells on lymphocyte proliferation in co-culture experiments. However, the expression of human leukocyte antigen-G decreased significantly in induced neuron-like cells. These results suggest that growth factor-based methods enable the differentiation of mesenchymal stem cell toward immature neuronal-like cells, which retain their immunomodulatory and anti-oxidative activities.The immunomodulatory and anti-oxidative activities of differentiated mesenchymal stem cells contribute to their therapeutic efficacy in cell-replacement therapy. Mesenchymal stem cells were isolated from human umbilical cord and induced to differentiate with basic fibroblast growth factor, nerve growth factor, epidermal growth factor, brain-derived neurotrophic factor and forskolin. The mesenchymal stem cells became rounded with long processes and expressed the neural markers, Tujl, neurofilament 200, microtubule-associated protein-2 and neuron-specific enolase. Nestin expression was significantly reduced after neural induction. The expression of immunoregulatory and anti-oxidative genes was largely unchanged prior to and after neural induction in mesenchymal stem cells. There was no significant difference in the effects of control and induced mesenchymal stem cells on lymphocyte proliferation in co-culture experiments. However, the expression of human leukocyte antigen-G decreased significantly in induced neuron-like cells. These results suggest that growth factor-based methods enable the differentiation of mesenchymal stem cell toward immature neuronal-like cells, which retain their immunomodulatory and anti-oxidative activities.

关 键 词：umbilical cord mesenchymal stem cell IMMUNOMODULATION oxidative stress neural induction neural regeneration 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]