5-氮胞苷诱导骨髓间充质干细胞向心肌样细胞的转化  

Mesenchymal stem cells transformed to myogenic cell induced by 5-azacytidine in vitro

作　　者：周亚峰[1] 李红霞[1] 程铖[1] 韩莲花[1] 杨向军[1] 

机构地区：[1]苏州大学附属第一医院心内科,江苏省苏州市215006

出　　处：《中国组织工程研究》2012年第49期9134-9138,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81170174);国家自然科学基金项目(81070139);江苏省自然科学基金项目(BK2011304);江苏省"科教兴卫"医学重点人才项目(RC2011111)~~

摘　　要：背景:利用骨髓间充质干细胞多向分化潜能及其可在体外表达多种外源目的基因的特性,将其作为种子细胞用于未来组织器官的修复和替代已成为当前研究的热点。目前,已从人和几种动物骨髓中分离出间充质干细胞,但对猪骨髓间充质干细胞的研究报道很少。目的:建立猪骨髓间充质干细胞体外培养及其转化为心肌样细胞的方法,并探讨其生物学特性。方法:猪的骨髓间充质干细胞分离纯化后,加入5-氮胞苷诱导分化,用抗desmin、MHC、cTnI、Cx-43进行免疫细胞化学染色鉴定。结果与结论:体外培养的猪骨髓间充质干细胞经5-氮胞苷诱导后,部分间充质干细胞呈梭形,阳性表达desmin、MHC、cTnI和Cx-43。结果提示猪骨髓间充质干细胞在体外条件下经5-氮胞苷诱导后可分化为心肌样细胞。BACKGROUND： With the characteristics of multiple differentiation potential and the in vitro expression of a variety of exogenous genes, bone marrow mesenchymal stem cells are used as the seed cells for the future repairing and replacing of tissues and organs and the research has become the hot spot. To date, bone marrow mesenchymal stem cells have been isolated from human and several kinds of animals, but reports on porcine bone marrow mesenchymal stem cells are rare. OBJECTIVE： To establish a method for culturing porcine bone marrow mesenchymal stem cells and then transformation into myogenic cells in vitro, and to investigate the underlying biological mechanisms. METHODS： Porcine bone marrow mesenchymal stem cells were isolated and purified by centrifugation. After induced with 5-azacytidine, the immunohistochemical staining was performed with desmin, MHC, cTnl and Cx-43. RESULTS AND CONCLUSION： After induced with 5-azacytidine, some porcine bone marrow mesenchymal stem cells became spindle-like and were positive for desmin, MHC, cTnl and Cx-43 expression. Porcine bone marrow mesenchymal stem cells can be transformed into myogenic cells after in vitro induced with 5-azacytidine.

关 键 词：心肌样细胞 骨髓间充质干细胞 5-氮胞苷 诱导分化 免疫细胞化学染色 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]