骨髓间充质干细胞修复Wistar大鼠的脊髓损伤  

Bone marrow mesenchymal stem cells repair Wistar rat spinal cord injury

作　　者：贾全章[1] 李东君[2] 陈玉丙[3] 孙景海[1] 王风华[1] 徐爽[1] 刘丽萍[3] 高德萱[2] 姜大伟[2] 

机构地区：[1]解放军第208医院,吉林省长春市130062 [2]延边大学医学部,吉林省延吉市133002 [3]吉林大学附属第二医院,吉林省长春市130041

出　　处：《中国组织工程研究》2012年第49期9209-9213,共5页Chinese Journal of Tissue Engineering Research

基　　金：吉林省科技发展计划项目(20080446)~~

摘　　要：背景:研究表明,骨髓间充质干细胞移植治疗脊髓损伤已取得疗效。目的:观察骨髓间充质干细胞移植对脊髓损伤大鼠脊髓功能的影响。方法:120只大鼠随机数字表法分为4组:空白组不造模,于脊髓损伤后当天损伤区局部注射生理盐水10μL;模型组采用改良的Allen’s打击法造成脊髓损伤模型,不做任何治疗;低浓度治疗组行脊髓损伤后当天经尾静脉注射浓度为1×109L-1骨髓间充质干细胞1mL;高浓度治疗组行脊髓损伤后当天损伤区局部注射浓度为4×1010L-1骨髓间充质干细胞10μL。结果与结论:损伤后1d模型组和两治疗组均无恢复迹象,运动评分低于空白组(P<0.01);损伤7d,两治疗组运动功能有所恢复,行为学评分均高于模型组,但差异无显著性意义(P>0.05);损伤15,30d,两治疗组行为学评分均明显高于模型组(P<0.01)。移植后7,15,30d脊髓病理切片显示治疗组较模型组均有显著恢复。提示骨髓间充质干细胞对脊髓损伤模型大鼠的脊髓功能有修复作用。BACKGROUND： Studies have shown that bone marrow mesenchymal stem cells transplantation has achieved therapeutic effects in the treatment of spinal cord injury. OBJECTIVE： To investigate the effects of bone marrow mesenchymal stem cells transplantation on motor function of rats with spinal cord injury. METHODS： 120 Wistar rats were randomly divided into four groups： （1） in the blank group, rats were not modeled but they were locally injected with 10 μL normal saline at the day of spinal cord injury; （2） in the model group, spinal cord injury models were established by modified Allen＇s crack method without any treatment; （3） in the low dose treatment group, 1 mL bone marrow mesenchymal stem cells （1 ×10^9/L） were injected through the tail vein at the day of spinal cord injury; in the high dose treatment group, rats were locally injected with 10 μL bone marrow mesenchymal stem cells （4×1^9/L） at the day of spinal cord injury. RESULTS AND CONCLUSION： At 1 day after injury, there was no healing in the model group and two treatment groups, and the scores of motion function in the three groups were lower than those in the blank group （P 〈 0.01）; at 7 days after injury, the scores of motion function in the two treatment groups were increased, and the behavior scores of the two treatment groups were higher than those of the model group, but there was no significant difference （P 〉 0.05） at 15 and 30 days after injury, the behavior scores of the two treatment groups were higher than those of the model group （P 〈 0.01 ）. At 7, 15 and 30 days after transplantation, there was significant recovery in behaviors in the two treatment groups compared with the model group, it indicates that bone marrow mesenchymal stem cells have therapeutic effects on the spinal function of rats with spinal cord injury.

关 键 词：骨髓间充质干细胞 修复 脊髓损伤 细胞移植 大鼠 静脉移植 局部注射 细胞浓度 干细胞 运动功能 

分 类 号：R394.2[医药卫生—医学遗传学]