EGF对原发性开角型青光眼小梁网细胞增殖和凋亡的影响  

Effect of Epidermal Growth Factor on Proliferation and Apoptosis of Cultured Trabecullar Meshwork Cells in Primary Open-Angle Glau- coma Patients

作　　者：陈燕萍[1] 吴瑜瑜[1] 郭茂生[1] 梁宗宝[1] 

机构地区：[1]福建医科大学附属第二医院福建省,泉州市362000

出　　处：《医学分子生物学杂志》2012年第4期267-271,共5页Journal of Medical Molecular Biology

基　　金：泉州市科技计划项目专项经费（No.2010232）

摘　　要：目的探讨EGF对体外培养POAG小梁网细胞增殖和凋亡的影响。方法体外培养POAG小梁网细胞并鉴定；用终浓度为0，5，10，20，50，100ng／ml的EGF干预48h，运用CCK-8比色法检测其吸光度值、流式细胞仪检测其凋亡率。结果成功进行POAG小梁网的培养和鉴定；CCK-8比色法结果表明，当EGF的浓度为5，10，20，50，100ng／ml时，其增殖率分别为13．8％、21．7％、26．5％、34．5％、17．0％，与对照组比较，差异具有统计学意义（P〈0．05）；流式细胞仪检测结果显示，其凋亡率分别为（16．14±0．44）％，（14．22±0．18）％，（10．90±0．49）％，（5．98±0．14）％，（5．58±0．21）％，（9．93±0．17）％，各实验组与阴性对照组比较，凋亡率下降具有统计学意义（P〈0．01）。结论EGF可以促进体外培养的POAG小梁网细胞的增殖，减少其凋亡。Objective To investigate the effects of epidermal growth factor （EGF） on the pro- liferation and apoptosis of human trabecullar meshwork cells （HTCs） . Methods HTCs of patients with primary open-angle glaucoma （POAG） were primarily cultured in vitro and identified by trans- mission electron microscopy and immunocytochemistry. The 5th-passage cells were incubated with different concentrations of EGF （0, 5, 10, 20, 50, 100 ng/mL） for 48 h respectively. The pro- liferation of HTCs was detected by Cell Counting Kit-8 assay and the apoptosis of HTCs determined by flow cytometry. Results HTCs were successfully cultured in vitro. They profoundly proliferated after the EGF treatment. The apoptotic HTCs were significantly decreased after EGF treatment for 48 h as compared with control groups （P 〈 0. 01 ） . Conclusion EGF can promote the proliferation of POAG trabecullar meshwork cells and inhibit their apoptosis.

关 键 词：青光眼 开角型 小梁网 表皮生长因子 

分 类 号：R775[医药卫生—眼科]