实际指数脉冲电场对细胞跨膜电位的影响  

Influence of Real Exponential Pulsed Electric Field on Transmembrane Potential for Cellular Membrane

作　　者：熊兰[1] 石岭岭[1] 郑家波[1] 谢子杰[1] 陈加鹏[1] 

机构地区：[1]重庆大学输配电装备及系统安全与新技术国家重点实验室,重庆400030

出　　处：《高电压技术》2013年第1期122-128,共7页High Voltage Engineering

基　　金：国家自然科学基金(51077139)~~

摘　　要：为了进一步增大细胞跨膜电位峰值,提高脉冲电场电穿孔效率,基于相关文献中球形单细胞多层介电模型的细胞跨膜电位计算方法,分析了实际RC指数衰减脉冲上升沿时间对细胞跨膜电位的影响。通过Matlab软件对细胞跨膜电位的传递函数频率响应进行了仿真分析,结果表明:不同频段的电场对细胞跨膜电位的影响不同。建立了一种改进的RC指数衰减脉冲模型,并进行了仿真计算,结果表明:脉冲电场上升沿时间对细胞跨膜电位有很大影响:上升沿时间越小,频率响应就越高,减小上升沿时间可以提高细胞跨膜电位峰值。仿真结论对改进脉冲电场发生电路的结构和参数设计,以增大细胞跨膜电位峰值并提高电穿孔效率提供了理论依据。In order to further increase the transmembrane potential and electroporation ratio, we analyzed the influence of different rising edges of real exponential decaying RC pulses on transmembrane potential by simulative calculation. The calculation was performed by using a classic multi-layer dielectric model of a spherical biological cell and its calculation method of transmernbrane potential. Simulative results from the freqtuency response of transmembranc potential transfer function in the software Matlab showed that electric fields at different frequency bands had different effects on transmembrane potential. Moreover, an improved exponential decaying RC pulse model was established and adopted in simulation. According to the results, transmembrane potential is closely related to the rising edge： both the frequency response and the magnitude of transmembrane potential increase with shorter rising time. The simulation results provide a theoretical guidance for enhancing the transmembrane potential and electroporation ratio by optimizing the pulsed electric field generation circuit.

关 键 词：脉冲电场 上升沿 跨膜电位 电穿孔 球形单细胞 多层介电模型 

分 类 号：Q27[生物学—细胞生物学]