玻璃化法超低温保存对大叶藻种子活力的影响  

Effects of cryopreservation by vitrification on the viability of eelgrass Zostera marina seeds

作　　者：张凌宇[1] 张沛东[1] 田璐[1] 孙燕[1] 张秀梅[1] 

机构地区：[1]中国海洋大学水产学院,山东青岛266003

出　　处：《生态学杂志》2013年第2期501-506,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(30400615);山东省优秀中青年科学家奖励基金项目(BS2011HZ017);海洋公益性行业科研专项(2013418043)资助

摘　　要：通过设置玻璃化法超低温保存的3个关键要素,即冰冻保护液处理、玻璃化溶液脱水时间和冷冻处理,研究了玻璃化法超低温保存对大叶藻种子活力的影响。结果表明:冰冻保护液处理和玻璃化溶液脱水时间对大叶藻种子活力无显著影响;冷冻处理组种子活力显著低于对照组,而冰冻保护液和玻璃化脱水处理对冷冻后种子活力的影响不显著,表明冰冻保护液和玻璃化溶液未对种子起到明显的保护作用;未冰冻保护液处理组玻璃化溶液脱水4h时种子活力显著低于其他脱水时间,而冰冻保护液处理组脱水4h时与其他脱水时间无明显不同,表明较长时间的玻璃化溶液脱水处理需使用冰冻保护液进行预处理。研究结果为超低温保存大叶藻种子提供了参考。In this paper, three key factors of cryopreservation by vitrification, i. e. , cryoprotec- tive solution application, dehydration time of vitrification solution, and rapid freezing, were installed to study the effects of cryopreservation by vitrification on the viability of Zostera marina seeds. Cryoprotective solution application and dehydration time had no significant effects on the viability of the seeds. When exposed to rapid freezing, the viability of the seeds was significantly lower than that of the control, while cryoprotective solution application and dehydration time showed no significant protective effects on the viability of the seeds. Without cryoprotective solu- tion application, the viability of the seeds subjected to 4 h dehydration was significantly lower than that of the seeds subjected to other lengths of dehydration time. With cryoprotective solution application, no significant difference was found between the viability of the seeds subjected to 4 h dehydration and that of the seeds subjected to other lengths of dehydration time. These findings indicated the necessity of cryoprotective solution application to seeds prior to the long period dehydration by vitrification. This study provides a reference for the cryopreservation of Z. marina seeds.

关 键 词：大叶藻 种子 超低温保存 玻璃化 生活力 

分 类 号：Q945[生物学—植物学]