沉默CHOP基因减轻脑缺血再灌注大鼠的急性脑损伤  

Silencing CHOP Gene gene Reduced reduced The the Acute acute Brain brain Injury injury in Cerebral cerebral Ischemia ischemia Reperfusion reperfusion in rRats

作　　者：郑胜哲[1] 谷月[2] 何春珂 王婷婷 贾丽君[5] 

机构地区：[1]延边大学附属医院神经内科,吉林延吉133000 [2]吉林大学白求恩第一医院呼吸科,吉林长春130021 [3]吉林省中西医结合医院骨科,吉林长春130021 [4]淄博市第一医院感染病科,山东淄博255200 [5]长春解放军第208医院神经内科,吉林长春130062

出　　处：《中风与神经疾病杂志》2013年第1期18-21,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究大鼠脑缺血再灌注后急性脑损伤中CHOP蛋白的作用。方法将36只SD大鼠随机分为对照组、vector组和LV-shRNA组,经微型注射泵向control组左脑室中注射PBS,vector组注射入LV-CMV-control质粒,向LV-shRNA组注射入LV-CMV-CHOP shRNA质粒。线栓法制作大鼠缺血再灌注模型后,TTC染色检测各组大鼠脑梗死体积,Western Blot检测所有大鼠脑内CHOP、Bcl-2和Caspase 3的表达,TUNEL染色检测梗死区细胞凋亡。结果 LV-shRNA组大鼠脑梗死体积明显小于对照组和vector组(P<0.01);Western Blot结果显示LV-shRNA组大鼠脑内CHOP和Caspase 3含量明显低于对照组和vector组(P<0.01),而Bcl-2高于对照组和vector组(P>0.05);TUNEL染色显示LV-shRNA组大鼠脑梗死区域内凋亡细胞明显少于对照组和vector组(P<0.01)。结论CHOP在脑缺血后具有促进急性脑损伤的作用,沉默CHOP可通过减轻细胞凋亡反应发挥神经保护作用。Objective ive To investigate the role of CHOP in the acute cerebral injury in rats with focal cerebral is- chemia/reperfusion. Methods 36 rats were randomly divided into control group,vector group and LV-shRNA group. PBS was injected into the left ventricle of rats in control group via micro-injection pump, whereas LV-CMV-control plasmid and LV-CMV-CHOP shRNA plasmid were injected into the rats brain of vector group and LV-CMV-control group respectively. The animal model of the left MCA ischemia/reperfusion was established by suture method. Cerebral infarct volume was measured by TTC staining and the expression of CHOP,Bcl-2 and Caspase 3 in rats brain were detected by Western Blot- blot. In addition,TUNEL staining was utilized to examine cell apoptosis in infarct zone. Results The infarction volume of rats in LV-shRNA group was obviously less than control group and vector group（ P 〈 0.01 ） . Western Blot blot showed that CHOP and Caspase 3 expression in LV-shRNA group rats brain were significantly lower than the control group and vec- tor group（ P 〈 0.01 ） ,while Bcl-2 was higher than those in the control group and vector group（ P 〉 0.05 ）. TUNEL staining showed that the apoptosis cells in LV-shRNA group rats＇ infarction area was markedly less than those in control groups and vector group （ P 〈 0.01 ）. Conclusion CHOP played plays a key role in promoting the acute brain injury after cerebral is- chemia. CHOP silencing could effectively reduce the apoptosis reaction and thus represented a neuroprotective function.

关 键 词：C EBP同源性蛋白 脑缺血再灌注 内质网应激 凋亡 

分 类 号：R743.3[医药卫生—神经病学与精神病学]