优选牵正膏薄层色谱鉴别方法  

Optimization of TLC Identification Method of Qianzheng Paste

作　　者：张文鑫[1] 耿燕娜[2] 段晓颖[1] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000 [2]河南大学淮河医院,河南开封475000

出　　处：《中医学报》2013年第2期231-232,共2页Acta Chinese Medicine

摘　　要：目的:优选牵正膏的薄层色谱鉴别方法。方法:运用不同的样品制备方法,比较薄层分离斑点的清晰度,从而确定更好的薄层色谱鉴别方法。结果:采用供试品4的制备方法制得的样品,薄层分离度高,斑点清晰。具体方法为10 g牵正膏置研钵中,加入适量硅藻土分散研碎,加入30 mL甲醇,2 mL盐酸,超声提取30 min,滤过,续滤液蒸干,加甲醇2 mL溶解残渣。结论:此方法简便、可靠、专属性好,可用作该制剂的质量控制方法。Objective： To discuss the optimization of thin layer chromatography（TLC） identification method of Qianzheng paste.Methods： Different sample preparation methods were used to compare the clarity of thin layer separation spots and to determine the better identification method of TLC.Results： The sample obtained by using the preparation method of sample 4 had a high thin layer separating degree and clear spots.Specific methods were as follows： 10 g of Qianzheng paste was put in mortar,appropriate diatomite was added to crush dispersively,30 mL methanol and 2 mL hydrochloric acid were added and ultrasonic extraction was conducted for 30 min for filtration and evaporation to dryness of continuous filtrate,and then 2 mL methanol was added to dissolve residue.Conclusion： This method is simple,reliable with good specificity,which can be used for quality control method of this preparation.

关 键 词：牵正膏 薄层色谱 五倍子 没食子酸 

分 类 号：R284.2[医药卫生—中药学]