金线莲中腺嘌呤的提取与分析  

Analysis of the extraction of adenine from Anoectochilus roxburghi

作　　者：吴萍萍[1] 黄丽英[1] 黄丽萍[1] 王美华[1] 陈晓兰[1] 

机构地区：[1]福建医科大学药学院,福建福州350004

出　　处：《中国医院药学杂志》2013年第3期215-218,共4页Chinese Journal of Hospital Pharmacy

基　　金：福建省科技厅重点项目(编号:2009I0009);福建医科大学教授基金项目(编号:01601086)

摘　　要：目的:应用超声-微波协同萃取技术提取金线莲中的腺嘌呤,高效液相色谱法-电喷雾电离/离子阱质谱法(HPLC-ESI/MS)对提取物中腺嘌呤进行测定和鉴别。方法:色谱条件为Agilent TC-C18色谱柱(5μm,4.6mm×250mm),流动相为甲醇∶0.02mol.L-1醋酸-醋酸铵缓冲溶液(5∶95,pH 4.6),流速1.0mL.min-1,检测波长260nm,柱温:30℃。结果:表明腺嘌呤浓度在0.5～60.0μg.mL-1范围内呈良好线性关系,相关系数为0.999 9,回收率在95.4%～96.2%之间,相对标准偏差为0.45%,测得金线莲(产于广西)提取物中腺嘌呤含量为10.64μg.g-1,结论:该法方法操作简便,灵敏快速。迄今为止,首次报道金线莲中含有重要药理活性的物质腺嘌呤,为金线莲的药理和临床试验研究提供物质基础。OBJECTIVE To extract adenine from Anoectochilus roxburghii by ultrasonic-microwave assisted extraction （UMAE）,and to identify and determine adenine by high performance liquid chromatography electrospray ion traps mass spectrometry. METHODS The chromatographic separation was achieved on Agilent TC-C18 column （5 μm, 4. 6 mm x 250 mru i. d. ） using methanol-0. 02 mol.L -1 acetic acid ammonium acetate buffer solution（5：95, v/v） as the mobile phase at a flow rate of 1. 1） ml,.min -1, 260 nm as the detection wavelength. RESULTS The analyte showed good linear regression relationship be tween peak area and concentration in the range of 0.5 - 60. 0μg· mL-1 （r= 0. 999 9）. The recovery rate was 95.4% - 96. 2% with an RSD of 0. 45%. The content of adnine extracted from Anoectochilus roxburghii was 10. 64 μg.g-1. CONCLUSION The proposed method is simple, accurate and rapid. So far there is no report to indicate that A. Roxburghii contains this important bioactive compound. It provides a scientific basis for further study clinical test and pharmacology of A. roxburghii.

关 键 词：金线莲 液质联用 分析 腺嘌呤 

分 类 号：R914[医药卫生—药物化学]