芹菜素对宫颈癌U14细胞增殖抑制作用的实验研究  

Experimental study on the inhibiting effect of apigenin on the proliferation of cervical carcinoma U14 cell

作　　者：王文钊[1] 孙倩[1] 

机构地区：[1]天津市人民医院妇产科,300121

出　　处：《实用医学杂志》2013年第2期181-183,共3页The Journal of Practical Medicine

摘　　要：目的:探讨芹菜素对宫颈癌U14细胞增殖的抑制作用。方法:在昆明鼠腹腔孵育宫颈癌U14瘤株后,无菌抽取腹水,制成细胞悬液接种于培养瓶中,取对数生长期细胞用于实验。实验当日配制芹菜素液,浓度分别为0、20、40、80μmol/L,以MTT法检测不同浓度芹菜素分别在24、48、72h对宫颈癌U14细胞增殖活性的影响。结果:芹菜素(20～80μmol/L)能够抑制小鼠宫颈癌U14细胞增殖,并表现为时间、浓度的依赖性。结论:芹菜素在一定浓度下可抑制体外U14细胞增殖并有时间依赖性。Objective To explore the effect of apigenin inhibiting cervical carcinoma U14 cell proliferation. Method The cervical cancer U14 strains were incubated in abdominal cavity.of Kunming mice. The ascetic fluid was drawn in sterile condition and made the suspension cultured in flask. The logarithmic growth phase ceils were used for experiment. On the experimental day, the apigenin solution was made in the concentration of 0, 20, 40, 80 μmol/L. After 24, 48, 72 h, the effect of cellular proliferative activity was checked by MTr. Result The Apigenin solution （20 -80 μmol/L）could inhibit the cellular proliferation of U14 cells in concentration-and timedependent manners. Conclusion The certain concentration of Apigenin solution can inhibit the cellular proliferation of U14 cells in time-dependent manner.

关 键 词：芹菜素 宫颈癌U14 细胞增殖 

分 类 号：R737.33[医药卫生—肿瘤]