17-AAG对MCF-7细胞增殖的抑制作用及对STAT3和VEGF表达的影响  

17-AAG Inhibits Proliferation of MCF-7 Human Breast Cancer Cells and Down-regulates VEGF and STAT3 Expressions

作　　者：肖丽君[1] 赵恩宏[2] 赵爽[1] 高艺铭[1] 郭璐[1] 许倩[3] 

机构地区：[1]承德医学院免疫教研室,河北承德067020 [2]承德医学院附属医院肿瘤外科,河北承德067020 [3]承德医学院基础医学研究所,河北承德067020

出　　处：《中国医科大学学报》2013年第1期65-68,共4页Journal of China Medical University

基　　金：河北省科技厅科学技术项目(072761450);河北省教育厅自然科学项目(2007326);河北省人口和计划生育委员会科技项目(2011-A15)

摘　　要：目的观察17-AAG对人乳腺癌MCF-7细胞株STAT3、VEGFmRNA及蛋白表达水平的影响。方法体外培养MCF-7细胞，分别给予不同剂量及不同作用时间的17-AAG，采用四甲基偶氮唑盐微量酶反应比色法（MTT法）检测细胞增殖能力；RT-PCR和Westernblot法检测各组细胞STAT3和VEGFmRNA及蛋白的表达水平。结果0．165～10．000mg／L17-AAG作用24h、48h对MCF-7细胞有显著的抑制作用，且有明显的时间、剂量依赖性；1．0、2．0、3．0、5．0mg／L17．AAG作用48h后，各组细胞STAT3和VEGFmRNA及蛋白表达水平均下调，且具有浓度依赖性。结论17一AAG对人乳腺癌MCF．7细胞的增殖具有明显的抑制作用，并能抑制STAT3和VEGFmRNA及蛋白的表达，且具有剂量依赖性。Objective To investigate the effects of 17-AAG on VEGF and STAT3 expressions in human breast cancer MCF-7 cells. Methods MCF-7 cells were cultured in DMEM roediuro;MTr method was performed to evaluate the inhibitory effects of 17-AAG with dif- ferent time and different doses on proliferation of MCF-7 cells;the genes and proteins expressions of VEGF and STAT3 were determined by RT-PCR and Western blot analysis. Results With treatment of 0.165-10.000 mg/L 17-AAG for 24 hours and 48 hours, significantly inhibi- tion of the MCF-7 cells proliferation was observed in both time- and dose-dependent manner. The assay of RT-PCR and Western blot showed that the genes and proteins expressions of VEGF and STAT3 in MCF-7 cells induced by 17-AAG were significantly down-regulated in a dose-dependent manner when the cells were treated with 1.0,2.0,3.0,5.0 mg/L 17-AAG for 48 hours. Conclusion 17-AAG can in- hibit the cell proliferation and down regulate the genes and proteins expressions of VEGF and STAT3 of human breast cancer MCF-7 cells in vitro.

关 键 词：17-AAG 人乳腺癌MCF-7细胞 细胞增殖 VEGF STAT3 

分 类 号：R73[医药卫生—肿瘤]