产乙醇工程菌构建方法的研究进展  

Progress on the Construction Methods of Ethanol Engineering Strains

作　　者：常冬冬[1] 余志晟[1] 张洪勋[1] 

机构地区：[1]中国科学院研究生院资源与环境学院,北京100049

出　　处：《中国农学通报》2013年第2期120-124,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"纤维素废弃物热解液乙醇转化的工程菌构建"(21177153)

摘　　要：归纳了产乙醇工程菌的构建方法,包括原生质体融合技术、不同水平上的基因工程技术(包括经典基因工程、代谢工程、全局转录调控工程和进化工程)及低能离子束介导转基因技术,分析了各项技术的优缺点,指出低能离子束介导转基因技术在构建工程菌方面的巨大应用潜力,以期为构建能利用新型碳源发酵乙醇的工程菌提供思路和依据。In order to provide new ideas and basis for the construction of engineering strains which could ferment new carbon sources to ethanol,the construction methods concluding protoplast fusion technique,gene engineering technology of different levels （containing classic genetic engineering,metabolic engineering,global transcription machinery engineering and evolutionary engineering） and low-energy ion beam mediated transgenic technology were reviewed.The advantages and disadvantages of these technologies were also analyzed.The immense application prospect of low-energy ion beam mediated transgenic technology in engineering strains ＇ construction was shown in this paper.

关 键 词：乙醇 工程菌 原生质体融合 基因工程 低能离子束 

分 类 号：Q89[生物学]