脑源性神经营养因子对持续惊厥后海马凋亡调控基因表达的影响  

Effects of Brain-derived Neurotrophic Factor on Apoptotic Regulated Genes in Hippocampal Cells after Status Convulsivus

作　　者：苑爱云[1,2] 蒋莉[2] 侯梅[1] 李欣[2] 

机构地区：[1]青岛市妇女儿童医疗保健中心神经康复科,山东青岛市266034 [2]重庆医科大学附属儿童医院神经内科,重庆市400014

出　　处：《中国康复理论与实践》2013年第1期42-45,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(30271382)

摘　　要：目的观察外源性脑源性神经营养因子(BDNF)对惊厥持续状态(SC)后海马凋亡调控基因表达的影响。方法选用成年Wistar鼠32只制作氯化锂-匹罗卡品SC模型,另外32只为正常对照组。两组分别于一侧脑室内注射生理盐水、BDNF、抗BDNF抗体或不注射,于注射后6 h处死。免疫组化观察海马Bcl-2和c-Jun蛋白的表达;原位杂交和RT-PCR测定bcl-2和c-jun mRNA的表达。结果正常对照组双侧海马bcl-2及c-jun表达与同组无注射组比较无显著性差异。SC组注射侧海马bcl-2表达由高而低依次为:SC-BDNF组>SC-无注射组=SC-NS组(P<0.05)>SC-抗BDNF组(P<0.05),c-jun表达则呈相反变化趋势(P<0.05);SC组注射对侧海马bcl-2及c-jun表达与无注射对照组相比无显著性差异。结论 SC后,脑室内注射外源性BDNF可能通过上调凋亡抑制基因bcl-2、下调凋亡促进基因c-jun表达发挥其保护作用。脑室内注射BDNF在脑内扩散有限,在临床的应用受限。Objective To explore the effects of brain-derived neurotrophic factor （BDNF） on expression of apoptotic regulated genes （bcl-2 and c-jun） in hippocampus after status convulsivus （SC）. Methods Seizures were induced in 32 adult Wistar rats with lithium-pilocar- pine intraperitoneal injection （SC）, the other 32 rats were as the normal controls （NC）. The rats were sacrificed 6 h after injection of normal saline （NS）, BDNF, or anti-BDNF in left lateral ventricle （or no injection）. The expression of Bcl-2 and c-Jun protein and bcl-2 and c-jun mRNA were investigated with immunocytochemistry, RT-PCR and in situ hybridization. Results The expression of bcl-2 and c-jun （both protein and mRNA） was not significantly different in the hippocampus of both side in NC. In SC, the expression of bcl-2 ranged from more to less as BDNF〉NS and non-injection〉anti-BDNF in the hippocampus of non-injected side, while the expression of c-jun reversed. Howev- er, there was not significant difference in the expression of bcl-2 or c-jun in the hippocampus of non-injected side. Conclusion Exogenous BDNF can up-regulate the expression of bcl-2 and down-regulate the expression of c-jun in hippocampal cells, which may protect brain from apoptosis after after SC. The intraventricular injection of BDNF diffuses limited, which works less for clinical treatment.

关 键 词：惊厥持续状态 脑源性神经营养因子 凋亡 BCL-2 C-JUN 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]