光谱法研究夏枯草黄酮对酪氨酸酶的抑制作用  

Spectroscopic studies of inhibitory effect on tyrosinase of prunella vulgaris L.flavonoids

作　　者：闫家凯[1] 张国文[1] 周佳[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《分析试验室》2013年第2期17-21,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31060210);江西省自然科学基金(20114BAB204019);江西省教育厅科技项目(GJJ11287)资助

摘　　要：利用紫外-可见光谱法、荧光光谱法和圆二色谱法研究了夏枯草黄酮对酪氨酸酶的抑制作用及抑制机理。结果表明,夏枯草黄酮是一种可逆的竞争型酪氨酸酶抑制剂,且能很快地抑制酪氨酸酶的活性,其半抑制浓度(IC50)为79.25μg/mL,抑制常数(Ki)为130.33μg/mL。荧光光谱显示夏枯草黄酮能显著地增强酪氨酸酶的疏水性。圆二色谱分析表明夏枯草黄酮诱导酪氨酸酶的构象发生了部分改变。The inhibitory effect on tyrosinase and its inhibitory mechanism of Prunella vulgaris L. flavonoid as investigated by UV-vis absorption, fluorescence and circular dichroism （CD） spectroscopy. The result indicated that the Prunella vulgaris L. flavonoids was a reversible and competitive inhibitor, and the flawmoids couhl inhibit the activity of tyrosinase rapidly. The half inhibition concentration （IC50） of the flavonoids and inhibition constant （Ki） were obtained to be 79.25 μg/mL and 130. 33 μg/mL, respecuveiy, lne nuorescence pectta showed that the Prunella vulgaris L. flavonoids increased significantly the hydrophobicity of tyrosinase. The analysis of CD spectra indicated that the binding of the flavonoids to tyrosinase could induce conformational partial change in tyrosinase.

关 键 词：夏枯草黄酮 酪氨酸酶 抑制作用 光谱法 

分 类 号：O657.3[理学—分析化学]