Inhibitory effect of lanthanum chloride on migration and invasion of cervical cancer cells  

作　　者：陈宏伟 刘丝荪 缪丽芳 俞玲芳 汪泱 郭菲 

机构地区：[1]Burns Institute,The First Affiliated Hospital of Nanchang University [2]Postgraduate Department of Nanchang University [3]Department of Gynecology and Obstetrics,The First Affiliated Hospital of Nanchang University [4]Department of Gynecology and Obstetrics,Jiujiang Maternal and Children Health Hospital

出　　处：《Journal of Rare Earths》2013年第1期94-100,共7页稀土学报（英文版）

基　　金：Project supported by National Natural Science Foundation of China(30960405,81160193)

摘　　要：Tumor metastasis remains the main reason for treatment Jhilure and death of patients with cervical cancer. The prcsent study was designed to explore the effects of lanthanum chloride （LaCl3 on the invasion and migration of cervical cancel- cells and the underlying mechanisms. The migration and invasion of tumor cells was evaluated by a modified Transwell/Boyden chamber assays. It is well known that MMPs （Matrix metalloproteinases） and NF-κB （Nuclear factor-κB） pathway play important roles in migration and invasion of tumor cells, and also the expression of MMPs were regulated by NF-κB signaling. The expression on MMP-1 and MMP-9 was detected by reverse transcription polymerase chain reaction （RT-PCR）; Western blot and the NF-KB-DNA-binding ac tivity assay were used to analyze the NF-KB activity. The results indicated that LaCl3 was capable of inhibiting the cell invasion and migration of human cervical cancer Hela cells by decreasing the expression of MMP-1 and MMP-9 via blocking NF-κB pathwayTumor metastasis remains the main reason for treatment Jhilure and death of patients with cervical cancer. The prcsent study was designed to explore the effects of lanthanum chloride （LaCl3 on the invasion and migration of cervical cancel- cells and the underlying mechanisms. The migration and invasion of tumor cells was evaluated by a modified Transwell/Boyden chamber assays. It is well known that MMPs （Matrix metalloproteinases） and NF-κB （Nuclear factor-κB） pathway play important roles in migration and invasion of tumor cells, and also the expression of MMPs were regulated by NF-κB signaling. The expression on MMP-1 and MMP-9 was detected by reverse transcription polymerase chain reaction （RT-PCR）; Western blot and the NF-KB-DNA-binding ac tivity assay were used to analyze the NF-KB activity. The results indicated that LaCl3 was capable of inhibiting the cell invasion and migration of human cervical cancer Hela cells by decreasing the expression of MMP-1 and MMP-9 via blocking NF-κB pathway

关 键 词：lanthanum chloride （LaCl3） cervical cancer INVASION migration： NF-~B rare earths 

分 类 号：R737.33[医药卫生—肿瘤]