凝胶渗透色谱-高效液相色谱法测定植物油中苯并芘  

Determination of Benzo(a)pyrene in vegetable oil by GPC-HPLC

作　　者：刘艳琴[1] 王浩[1] 杨红梅[1] 

机构地区：[1]国家食品质量安全监督检验中心,北京100094

出　　处：《食品科技》2013年第1期327-329,共3页Food Science and Technology

摘　　要：建立了用凝胶渗透色谱-高效液相色谱法测定植物油中苯并芘的方法。该方法以乙酸乙酯-环己烷（1：1,v/v）提取样品,凝胶渗透色谱净化,以ZORBAXSB-C18柱（4.6mm×250mm,5μm）分离,流动相为甲醇和水（90＋10）,流速1.0mL/min,激发波长365nm,发射波长412nm,柱温25℃,进样量25μL。该方法的检出限0.4μg/kg,线性范围0.1~10.0μg/L,加标回收率98.7%~109.2%,相对标准偏差为3.81%~8.76%。This thesis developed a GPC-HPLC method for Benzo（a）pyrene in vegetable oil. In the method, sample was extracted with ethyl acetate/hexane （1：1, v/v）, purified by GPC, Chromatographic separation was carried out on a ZORBAX SB C 18 column（4.6 mm×250 mm, 5 μm）, the mobile phase was methanol：water（90：10）, the flow was 1.0 mL/min, exitcation wavelength was 365 nm, emission wavelength was 412 nm, the column temperature was 25 ℃, and the injection volume was 25 μL. The limit of detection was 0.4 μg/kg, The linear plots were obtained between 0.1~10.0 μg/L. Overall recoveries were between 98.7%~109.2%, and RSD value were between 3.81%~8.76%.

关 键 词：高效液相色谱 凝胶渗透色谱 苯并芘 植物油 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程]