千层塔内生真菌G324-4次生代谢产物化学成分及乙酰胆碱酯酶抑制活性研究  

Chemical Constituents from Endophytic Fungus G324-4 in Huperzia serrata and Their Anticholinesterase Activity

作　　者：李晓誉[1] 陈峰阳[1] 徐世芳[1] 叶益萍[1] 

机构地区：[1]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中华中医药学刊》2013年第1期89-90,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省钱江人才项目(2007R10033);浙江省医学重点学科群的资助项目(XKQ-010-001);浙江省科技计划项目(2009F20033)

摘　　要：目的:研究千层塔内生真菌G324-4次生代谢产物中的化学成分和抑制乙酰胆碱酯酶活性。方法:应用微生物培养的方法获得微生物菌丝体,再用溶剂提取,色谱方法分离化合物,并通过波谱方法鉴定其结构,Ellman法测定其乙酰胆碱酯酶抑制活性。结果:从千层塔内生真菌G324-4中得到2个化合物,分别鉴定为核丛青霉素(1)和Pencolide(2);核丛青霉素具有乙酰胆碱酯酶抑制活性,IC_(50)值为(7.30±0.52)μg/mL;Pencolide没有乙酰胆碱酯酶抑制活性。结论:千层塔内生真菌G324-4可代谢产生核丛青霉素和pencolide,有望成为新的药源微生物。Objective：To study the constituents from Endophytic Fungus G324 -4 in Huperzia serrata and their anticholinesterase activities.Methods：Constituents were extracted and isolated from the mycelia of G324 -4,and their structures were identified by spectroscopic data.Their anticholinesterase activities were measured with Ellman method.Results： Two compounds were identified as sclerotiorin（1） and pencolide（2）.Compound 1 possessed anticholinesterase activity.Conclusion：Endophytic Fungus G324-4 can metabolize Ssclerotiorin and pencolide,and it should be a new access for drugs.

关 键 词：千层塔 内生真菌 代谢产物 

分 类 号：R222.19[医药卫生—中医基础理论]