两种气滞血瘀证造模方法的比较  

Comparison of the Two Modeling Methonds for Qi Stagnation and Blood Stasis

作　　者：王婷婷[1] 贾乘[2] 宁天一[1] 姜静[1] 程嘉艺[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]沈阳医学院奉天医院,辽宁沈阳110024

出　　处：《中华中医药学刊》2013年第1期157-158,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金资助项目(20102143)

摘　　要：目的:比较两种大鼠气滞血瘀证模型的建立方法,为研究气滞血瘀病因病机提供实验基础。方法:分别采用声光电、夹尾、束缚、冰水浴多种刺激联合应用及肾上腺素注射的方法造模。采用MOTO压力传感技术检测血液流变学相关指标,采用凝固法检测凝血四项相关指标。结果:与空白对照组相比,多因素刺激组大鼠血液流变学指标中全血高、中、低切粘度与血浆黏度均显著升高(P<0.05),肾上腺素组大鼠全血高切粘度与血浆黏度显著升高(P<0.05);两组大鼠凝血四项指标中纤维蛋白原含量均显著升高(P<0.01)。结论:多因素刺激与肾上腺素注射法均能导致动物出现血瘀状态,肾上腺素注射法方便易行,多因素刺激组较符合中医病因病机理论。Objective：To compare methods of rat modeling for Qi stagnation and blood stasis for serving the experimental of etiological factors and pathogenesis.Methods：Apply various stimulations such as noise,light,electricity,tail-clamping, icewater-bath,bondage and adrenaline injection to establish the rat mode.Adopt the pressure sensing technology of MOTO to detect hemorheology-related indicators,use the coagulation method to detect coagulation-related indicators. Results：In hemorheology,compared with the negative control group,the viscosity of whole blood of high,medium,low shear and plasma viscosity increased in the multi -factor stimulation group（P0.05）.The viscosity of whole blood of high shear and plasma viscosity increased in the adrenaline injection group（P0.05）.The fibrinogen content in blood coagulation indicated significant increase in both group（P0.05 ）.Conclusion：Both multi-factor stimulation and adrenaline injection can lead to blood stasis.Adrenaline injection method can be convenient and easy.Multi-factor stimulation group relatively conform the theory of etiology and pathogenesis.

关 键 词：气滞血瘀 多因素 肾上腺素 血液流变学 凝血四项 

分 类 号：R-322[医药卫生]