姜黄素对乙醇诱导的人L-02肝细胞氧化损伤的保护作用研究  

Protective Effects of Curcumin on Ethanol Induced Oxidative Injury on Human L-02 Cell Line

作　　者：胡静[1] 陈新祥[1] 胡剑峰[1] 王洪武[2] 

机构地区：[1]湖北职业技术学院医学院,湖北省孝感市432000 [2]华中科技大学同济医学院附属同济医院感染科

出　　处：《医学理论与实践》2013年第1期3-4,7,共3页The Journal of Medical Theory and Practice

摘　　要：目的:探讨姜黄素对乙醇诱导的人L-02肝细胞氧化损伤的保护作用。方法:体外检测不同浓度梯度的姜黄素对人L-02肝细胞活力的影响;建立体外乙醇诱导的氧化损伤细胞模型,并以不同浓度姜黄素进行干预24h,检测肝细胞MDA水平、SOD和GSH-Px的活力。结果:与正常对照组相比,乙醇模型组MDA水平显著上升,SOD和GSH-Px活力明显下降(P<0.05),经姜黄素处理,MDA水平下降,SOD和GSH-Px活力显著上升(P<0.05)。结论:姜黄素可以减轻脂质过氧化程度,降低MDA含量,并提高肝细胞抗氧化的能力,使SOD与GSH-Px活性升高,发挥了对肝细胞的保护作用。Objective：To study protective effects of curcumin on ethanol induced oxidative injury on human L-02 cell line.Methods：The cell model on ethanol-induced oxidative injury was established by culturing L-02 cell with 200mmol/L ethanol in vivo for 24 hours as ethanol model groups.Half of ethanol group was treated with Curcumin at the same time as Curcumin-treated group.The SOD,GSH-Px and MDA in each group were detected.Results：The MDA in ethanol model group was significantly increased,but the activities of SOD and GSH-Px were decreased significantly than those in normal group respectively.After Curcumin intervention,the MDA was significantly decreased compared with the ethanol model group respectively.But the the activities of SOD and GSH-Px were increased significantly.Conclusion：Curcumin can protect ethanol induced oxidative injury in human L-02 cell line via alleviating lipid peroxidation and enhancing antioxidant abilities.

关 键 词：姜黄素 SOD活力 MDA含量 

分 类 号：R-33[医药卫生]