塞来昔布对人甲状腺髓样癌裸鼠种植瘤生长的抑制作用  

Inhibitory effect of celecoxib on growth of transplant tumor of medullary thyroid carcinoma in nude mice

作　　者：华特波[1] 孟宪瑛[1] 谢淑丽[1] 张强[1] 李群[1] 

机构地区：[1]吉林大学第一医院甲状腺外科,长春130021

出　　处：《中华实验外科杂志》2013年第1期49-51,共3页Chinese Journal of Experimental Surgery

基　　金：吉林省卫生厅科研课题（20082017）

摘　　要：目的观察塞来昔布对人甲状腺髓样癌裸鼠皮下种植瘤生长的抑制作用，并探讨其作用机制。方法按照人甲状腺髓样癌TT细胞2×10^6个／只接种在40只BALB／c裸鼠皮下制作成肿瘤动物模型，随机分成A、B、C、D4组，分别给予生理盐水、塞来昔布、阿霉素、塞来昔布联合阿霉素，其中塞来昔布每只300mg／（kg·d），阿霉素每只2mg／（kg·），待接种部位出现直径约0．5cm瘤结节后连续给予相应药物治疗1周，分别测量裸鼠体质量、肿瘤质量，3周后处死裸鼠取瘤体计算抑瘤率，采用免疫组织化学法检测肿瘤组织及肝、肺组织中环氧合酶-2（COX-2）、基质金属蛋白酶-9（MMP-9）、血管内皮生长因子（VEGF）的表达。结果A、B、C、D组的抑瘤率分别为0％、45．37％、49．71％、69．68％，B、C、D组与A组比较（P〈0．05），其余各组问抑瘤率比较差异无统计学意义（P〉0．05）。COX-2、MMP-9、VEGF在A组中的表达明显高于B、C、D组，差异有统计学意义（P〈0．05），三者在B、C、D组中的表达差异均无统计学意义（P〉0．05）。在各实验组肝组织中均出现肿瘤细胞的侵袭，COX-2、MMP-9、VEGF均呈现阳性。结论塞来昔布能抑制甲状腺髓样癌皮下种植瘤的生长，联合阿霉素能增强抑瘤效果。Objective To observe the inhibitory effect of celecoxib on the growth of transplant tumor of medullary thyroid carcinoma in nude mice. Methods Forty mice were randomly divided into 4 groups （ A, B, C and D） ： control group, celecoxib group, doxorubicin group and celecoxib ＋ doxorubicin group. All vaccination animal models were established by subcutaneous injection of 2 ×10^6 of human medul- lary thyroid carcinoma 3T cells. The celecoxib was given 300 mg/（kg·d）, and doxorubiein 2 mg/（kg·d）. Tumor volumes and weight of mice were measured one week after administration when 0. 5 cm tumor nodus appeared in the injection region, and tumor inhibition rate was calculated after 3 weeks. The expression levels of cyclooxegenase-2 （COX-2）, matrix metalloproteinases-9 （MMP-9） and vascular endothelial growth factor （VEGF） in the vaccination tumor, liver and lung tissues were detected by using immunohistochemistry. Results The inhibition rate in groups A, B, C, and D was 0%, 45.37%, 49. 71%, and 69. 68% respectively, and the inhibitory effects in groups B, C and D were increased as compared with group A （ P 〈 0. 05 ）, but there was no obvious difference among groups B, C and D. The expression levels of COX-2, MMP-9 and VEGF in group A were obviously higher than in groups B, C and D （P 〈 0. 05 ）, but there was no significant difference among groups B, C and D （ P 〉 0. 05 ）. In groups B, C and D, invasion of tumor cells in the liver and lung tissues was seen, and the expression of COX-2, MMP-9 and VEGF was positive. Conclusion Celeeoxib can inhibit the growth of vaccination tumors of medullary thyroid carcinoma, and combination of celecoxib with doxorubicin can exert the synergistic effects.

关 键 词：塞来昔布 甲状腺癌 环氧化酶-2 基质金属蛋白酶-9 血管内皮生长因子 

分 类 号：R73[医药卫生—肿瘤]