胚胎干细胞关键因子Nanog在人胶质瘤中的表达及意义  

Expression and its significance of embryonic stem cell-associated factor Nanog in human gliomas

作　　者：郭雨霁[1] 邴鲁军[1] 刘尚明[1] 郝爱军[1] 

机构地区：[1]山东大学医学院组织学胚胎学教研室,实验畸形学教育部重点实验室,济南250012

出　　处：《解剖学报》2013年第1期1-5,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(81100919);山东省优秀科学家奖励基金资助项目(BS2010Y041)

摘　　要：目的探讨胚胎干细胞关键因子Nanog在人胶质瘤中的表达及意义。方法通过免疫荧光双标技术,分析40例胶质瘤组织内胚胎干细胞关键因子Nanog与肿瘤干细胞相关基因Nestin、CD133及胚胎干细胞全能性相关基因Oct4的共表达情况,并通过RT-PCR、Western blotting技术分析胶质瘤组织内Nanog与脑胶质瘤恶性程度之间的关系。结果 Nanog在胶质瘤组织中的表达随着胶质瘤病理级别增高而升高,而且超过50%的Nanog阳性细胞同时表达Nestin和CD133。95%以上的Nanog阳性细胞都同时表达胚胎干细胞全能性相关基因Oct4。结论胶质瘤组织中Nanog多表达在肿瘤干细胞中,与胶质瘤的恶性程度呈正相关,在胶质瘤的发生、发展过程中起着重要作用,为研究胶质瘤的起源及胶质瘤的诊断和预后判断提供帮助。Objective To explore the expression of embryonic stem cell-associated factor Nanog in human gliomas and its significance. Methods Forty cases of human gliomas were examined. The expression of Nanog was analysed by immunohistochemistry, reverse transcription polymerase chain reaction and Western blotting. Double immunofluorescence staining was used to investigate the coexpression of Nanog and markers of cancer stem ceils or embryonic stem cell pluripotency-related gene Oct4. Results We found a positive correlation between the expression levels of Nanog and tumour malignancy. Immunohistochemistry showed that Nanogexpressed in both the nuclei and the cytoplasm of glioma cells. Double immunofluorescence staining revealed that more than 50% of Nanog-positive ceils coexpressed the putative CSC markers CD133 and Nestin. More than 95% Nanog positive cells also expressed embryonic stem cell pluripotencyrelated gene Oct4. Conclusion The result suggests that Nanog is mainly expressed in cancer stem ceils in gliomas and positively correlated with the malignancy of gliomas. Nanog may play an important role in the progress of gliomas.

关 键 词：胶质瘤 肿瘤干细胞 NANOG 免疫荧光 人 

分 类 号：R739.4[医药卫生—肿瘤]