洛伐他汀对大鼠心脏成纤维细胞多聚ADP核糖合成酶-1活性及表达的影响  

Suppressive effects of lovastatin on PARP-1 activation and expression in cultured rat cardiac fibroblast

作　　者：程溪[1] 黄丹[2] 王妍[2] 黄恺[2] 

机构地区：[1]华中科技大学附属协和医院风湿免疫科,武汉430022 [2]华中科技大学附属协和医院心内科

出　　处：《临床心血管病杂志》2013年第2期149-151,共3页Journal of Clinical Cardiology

摘　　要：目的:观察洛伐他汀对大鼠心脏成纤维细胞多聚ADP核糖合成酶(PARP)-1活性及表达的影响。方法:采用PARP活性检测试剂盒和Western blot法检测洛伐他汀作用前后细胞内PARP-1的活性和蛋白表达水平。结果:使用洛伐他汀后,PARP-1的活性和蛋白表达均下调;再加用甲羟戊酸后,PARP-1的活性和表达下调被逆转。结论:洛伐他汀不仅可抑制血管紧张素Ⅱ刺激的心脏成纤维细胞PARP-1的活性及表达,还可在基础状态下抑制PARP-1的活性及表达,以上抑制作用都可以被甲羟戊酸逆转。Objective：To explore the effect of lovastatin treatment on activity and expression of poly （ADP- ribose） polymerase （PARP）-1 in cultured cardiac fibroblast. Method：The activity and expression of PARP-1 were measured by universal colorimetric PARP assay kit and Western blot assay. Result： The activity and expression of PARP-1 were down-regulated by lovastatin treatment. And this response could be reversed by addition of mevalonate. Conclusion：Lovastatin can obviously inhibit the activity and expression of PARP-1 induced by Ang II and under the normal condition. And these suppressive effects can be reversed by mevalonate.

关 键 词：血管紧张素Ⅱ 心脏成纤维细胞 洛伐他汀 甲羟戊酸 多聚ADP核糖合成酶 

分 类 号：R541[医药卫生—心血管疾病]