电解质水对粪肠球菌生物膜的杀菌作用研究  

The bactericidal efficiency of electrolyzed water on Enterococcus faecalis biofilm

作　　者：赵春苗[1] 程小刚[1] 范晓敏[1] 任世荣 白玉[1] 于丁一[1] 余擎[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]宝鸡烽火集团,陕西宝鸡721006

出　　处：《牙体牙髓牙周病学杂志》2013年第1期17-20,5,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81170948)

摘　　要：目的:观察强酸性电解质水(SAEW)和碱性电解质水(AEW)对粪肠球菌生物膜的杀菌作用。方法:利用BioFluxTM200微流体系统建立粪肠球菌生物膜模型,并分别用SAEW、AEW、52.5 g/L次氯酸钠液(阳性对照)和生理盐水(阴性对照)处理1、5、10 min后,激光共聚焦显微镜(CLSM)观察并采集荧光图像;ImageJ图像分析软件分析并计算各处理组不同时间点的活菌比例。结果:SAEW、AEW和52.5 g/L次氯酸钠液处理1、5、10 min各时间点的活菌比例都显著低于生理盐水组(P<0.05),其中AEW组活菌比例下降幅度最小,与SAEW和52.5 g/L次氯酸钠液处理组相比,各时间点均有统计学差异(P<0.05);而SAEW与52.5 g/L次氯酸钠液处理组相比,各时间点的活菌比例均无统计学差异(P>0.05)。结论:SAEW和AEW对粪肠球菌均有杀菌作用,其中SAEW的作用与52.5 g/L次氯酸钠液相当;而AEW的杀菌效果较弱,且需要延长作用时间。AIM： To evaluate the bactericidal efficiency of strong acid electrolyzed water （SAEW） and al- kaline electrolyzed water （AEW） on Enterococcusfaecalis biofilm. METHODS： The Enterococcusfaecalis dilution was added to the BioFluxTM 200 system to form biofilm. The biofilm was then treated with SAEW and AEW for 1 min, 5 min and 10min respectively. 52.5 g/L NaC10 and normal saline （NS） were used as the positive and negative controls. Confocal laser scanning microscopy （CLSM） was used to evaluate the bactericidal efficiencies. ImageJ was used to ana- lyze the fluorescence images. RESULTS ： At all time points, the survival rates of Enterococcus faecalis in SAEW and AEW groups were significantly lower than that in NS group （ P 〈 0.05 ）. No difference was found between SAEW group and 52.5 g/L NaC10 or the positive control group （P 〉 0.05）. However, AEW group showed higher survival rate than SAEW and 52.5 g/L NaC10 groups （P 〈 0.05 ）. CONCLUSION： Both SAEW and AEW showed bactericidal effects on Enterococcus faecalis. SAEW has similar bactericidal efficacy with NaC10, however the bactericidal efficiency of AEW was much weaker.

关 键 词：粪肠球菌 生物膜 微流体系统 强酸性电解质水 碱性电解质水 次氯酸钠液 

分 类 号：R780.2[医药卫生—口腔医学]