脂肪干细胞复合载杜仲醇提取物支架材料修复兔下颌骨缺损的实验研究  

Research on the adipose-derived stem cells combined with the extract of Eucommiol scaffold material to repair the rabbit mandible defect

作　　者：房殿吉[1] 郭延伟[1] 李松[1] 宁兆荣[1] 

机构地区：[1]佳木斯大学附属口腔医院颌面外科,佳木斯154000

出　　处：《华西口腔医学杂志》2013年第1期65-69,共5页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(81170935)

摘　　要：目的探讨杜仲醇提取物对脂肪干细胞成骨分化的促进作用及脂肪干细胞复合载杜仲醇提取物支架材料应用于颌骨缺损修复的可行性。方法将48只新西兰大白兔随机分为4组,并制备双侧下颌骨缺损模型。A组植入脂肪干细胞复合载杜仲醇提取物支架材料;B组植入脂肪干细胞复合羟磷灰石材料;C组单植入羟磷灰石材料;D组为空白组。分别于术后2、4、8、12周处死实验动物,制备组织标本,行大体观察、影像学分析、苏木精-伊红(HE)染色、扫描电镜(SEM)检测,并对影像学分析值及成骨情况进行评价,采用SPSS 17.0统计软件对数据进行分析。结果影像学检查及组织学染色显示A组骨缺损处愈合程度、成骨速度及其质量明显优于其他3组。SEM观察可见,A组复合材料与组织相容性好,无炎症刺激反应。统计牙CT分析数据及新生骨面积,A组的成骨情况优于其他3组(P<0.05)。结论杜仲醇提取物有促进脂肪干细胞向成骨细胞转化的作用,脂肪干细胞复合载杜仲醇提取物支架材料也具有明显骨诱导作用,是一种新型复合材料,可望成为临床应用中修复下颌骨缺损的新型材料。Objective To investigate the effect of Eucommiol on osteogenic differentiation of adipose-derived stem cells CADSCs）, and the feasibility of applying in mandibular defects repair using ADSCs combined with the extract of Eucommiol scaffold material. Methods Forty-eight New Zealand rabbits were randomly divided into four groups and bilateral mandibular defect was prepared. Group A： Implanted the ADSCs combined with the extract of Eucommiol scaffold, group B： Implanted the ADSCs combined with hydroxyapatite materials, group C： Implanted hydroxyapatite materials, group D： The control group. All the experimental animals were sacrificed after 2, 4, 8, 12 weeks. Tissue samples were observed by gross observation, radiographic analysis, hematoxylin-eosin（HE） staining, scanning electron microscope （SEM）. The value of imaging analysis and osteogenesis were evaluated. The results were analyzed by SPSS 17.0 statistical software. Results In vivo imaging and histological staining showed that the healing of bone defect and bone quality in group A was significantly better than those in the other groups. SEM showed well biocompatibility between composite material and tissue without inflammation reaction. By measuring and analyzing the dental CT data and new bone area, the bone in group A was obviously better than those in other groups （.P〈0.05）. Conclusion The extract of Eucommiol can promote ADSCs into bone cells. The role of ADSCs combined with extract of Eucommiol scaffold materials has significant bone induction. It is expected that this material could become a new composite ma- terial and be used to fix the jaw bone defects.

关 键 词：骨 下颌骨缺损 杜仲醇提取物 脂肪干细胞 羟磷灰石 

分 类 号：R782[医药卫生—口腔医学]