不同基因型变异链球菌临床分离株的分离和鉴定  

Isolation and identification of Streptococcus mutans strains with different genotype from clinical samples

作　　者：王成龙[1] 苏东华[1] 刘佼佼[2] 储冰峰[1] 李少华[3] 夏伟[3] 罗燕萍[4] 杨继勇[4] 丁红梅[3] 赵强[3] 邓斌[1] 席庆[1] 徐娟[1] 邵宁生[3] 

机构地区：[1]中国人民解放军总医院口腔科,北京100853 [2]沈阳军区总医院口腔科,沈阳110840 [3]军事医学科学院基础医学研究所,北京100850 [4]中国人民解放军总医院微生物科,北京100853

出　　处：《华西口腔医学杂志》2013年第1期80-85,共6页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30772426,81041028)

摘　　要：目的分离鉴定变异链球菌临床分离株。方法收集高龋患者和无龋健康人口腔中牙菌斑标本进行厌氧培养,通过细菌形态学观察、生物化学鉴定、自动微生物分析系统分析、聚合酶链反应(PCR)扩增变异链球菌基因的特异性片段表面蛋白抗原Ⅰ/Ⅱ(spaP)、葡糖基转移酶B(gtfB)和葡聚糖酶(dexA)以及基因分型等方法对获得的变异链球菌临床分离株进行鉴定。结果从32例临床受试者口腔中分离获得46株变异链球菌临床分离株,经生物化学,自动微生物分析系统,变异链球菌的特异性基因spaP、gtfB和dexA的PCR扩增均鉴定为变异链球菌,基因分型发现其中5株临床分离株的基因型与其他菌株相同。结论获得41株不同基因型变异链球菌临床分离株。Objective To identify Streptococcus mutans （S. mutans） strains from clinical samples. Methods Plaque samples from caries-active and caries-free sites on enamel surfaces were obtained and cultivated for S. mutans isolation. Morphology, biochemistry, automatic microorganism analysis system and polymerase chain reaction using primers homo- logous to surface protein antigen I/II （spaP）, glucosyhransferase B （gtfB） and dextranase （dexA） were used to identify S. mutans. Genotype of isolated S. mutans was determined by arbitrarily primed polymerase chain reaction. Results Forty-six strains of S. mutans were obtained from the 32 subjects and were identified as S. mutans by biochemistry, automatic microorganism analysis system and polymerase chain reaction. Five identical genotypes were found by arbi- trarily primed polymerase chain reaction. Conclusion Forty-one strains of S. mutans with different genotype were obtained from clinical samples.

关 键 词：变异链球菌 基因型 分离 鉴定 

分 类 号：R780.2[医药卫生—口腔医学]