大鼠脑缺血/再灌注后bFGF和GAP-43的表达与神经再生  

The expression of bFGF,GAP-43 and neurogenesis after cerebral ischemia/reperfusion in rats

作　　者：石旺清[1,2] 郑关毅[1] 陈晓东[1] 朱元贵[1] 张静[1] 江琼[1] 

机构地区：[1]福建医科大学附属协和医院,福州350001 [2]福建省莆田市第一医院,莆田351100

出　　处：《中国应用生理学杂志》2013年第1期63-67,I0002-I0004,共8页Chinese Journal of Applied Physiology

基　　金：福建省教育厅高等学校新世纪优秀人才支持项目(NCETFJ-0704);福建医科大学教授学术发展基金(JS09014)

摘　　要：目的:观察大鼠脑缺血/再灌注后不同时间段碱性成纤维生长因子(bFGF)和生长相关蛋白43(GAP-43)表达与神经元再生的变化,探讨其与神经再生的有关机制。方法:建立大鼠大脑中动脉阻塞模型(MCAO),并分为缺血再灌注3 d、7 d、14 d和28 d四组(n=6)。以神经损伤严重程度评分(NSS),运动评分测试(SMT)评估神经功能缺损程度,Nissl和TUNEL染色法观察不同时段缺血区周边组织神经元存活和凋亡情况,应用蛋白免疫印迹法和免疫荧光双标法检测缺血/再灌注后不同时段缺血区周边组织bFGF和GAP-43的表达水平和神经元再生的变化情况。结果:大鼠脑缺血/再灌注后3 d,大鼠出现了明显的神经功能缺损及运动功能障碍,缺血区周边组织神经元凋亡亦达到高峰,同时bFGF和GAP-43表达增强,7 d达到高峰,以后逐渐减弱,缺血周边组织可见散在的新生神经元,持续到28 d。结论:大鼠脑缺血/再灌注后内源性bFGF和GAP-43表达水平增加,可能与其神经修复和再生有关。Objective： To observe time points of the expressions of basic fibroblast growth factor（bFGF）,growth associated protein-43（GAP-43） and neurogenesis after cerebral ischemia/reperfusion in rats and explore its possible mechanism of neurogenesis.Methods： Models of middle cerebral artery occlusion（MCAO） were established in SD rats which were divided into 3 d,7 d,14 d and 28 d groups（n=6）.The neurological severity was evaluated by neurological severity scores（NSS） and scores of motor test（SMT）.Neuronal injury in the boundary zone of the infarction area was evaluated by TUNEL and Nissl staining;The expressions of bFGF and GAP-43 and neurogenesis were evaluated by Western blot and 5-bromodeoxyuridine（Brdu） fluorescence staining,respectively.Results： It showed up neurologic impairment and motor dysfunction after cerebral ischemia/reperfusion in rats at 3 d,the numbers of neuron apoptosis also peaked at 3d,the protein levels of bFGF and GAP-43 were significantly increased in timedependent manner,peaked at 7 d and then decreased gradually,meanwhile,Brdu and NeuN double fluorescence staining displayed scattered Brdu-and NeuN-positive cells in the boundary zone of the infarction area.Conclusion： These results suggest that the upregulation of bFGF and GAP-43 may contribute to the neurogenesis after cerebral ischemia/reperfusion.

关 键 词：脑缺血 神经元再生 碱性成纤维生长因子 生长相关蛋白43 

分 类 号：R743.3[医药卫生—神经病学与精神病学]