口腔鳞癌中转化生长因子β1 mRNA的表达及其与血管生成的关系  

Correlation between transforming growth factor-β1 mRNA expression and angiogenesis in oral squamous cell carcinoma

作　　者：孙乐刚[1] 刘玲[2] 王静静[1] 于婷婷[1] 宋宇峰[3] 

机构地区：[1]滨州医学院附属医院口腔外科,256603 [2]滨州医学院附属医院胃镜室,256603 [3]贵阳医学院附院口腔外科

出　　处：《实用口腔医学杂志》2013年第1期40-43,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目(编号:30660200)

摘　　要：目的:探讨口腔鳞癌中转化生长因子-β1(transforming growth factor-β1,TGF-β1)mRNA的表达及其与临床病理学特征和肿瘤血管生成的关系。方法:应用组织原位杂交和免疫组化检测48例口腔鳞癌组织和10例正常口腔黏膜组织中TGF-β1 mRNA表达,并计数微血管密度(MVD),用SPSS 13.0软件包中的t检验及χ2检验进行统计学分析。结果:TGF-β1mRNA定位于肿瘤细胞胞质,在口腔鳞癌中的表达明显高于正常口腔黏膜组织(P<0.01);TGF-β1 mRNA的表达与口腔鳞癌的浸润深度、淋巴结转移密切相关(P<0.01),而与患者的年龄、性别及病理分级无关;TGF-β1 mRNA的表达与MVD值呈正相关(P<0.01)。结论:TGF-β1 mRNA的高表达可能在口腔鳞癌的浸润和淋巴结转移过程中起重要作用,并与肿瘤血管生成有关。Objective： To investigate the correlation between transforming growth factor-β1 （TGF-β1 ） gene expression and clinico- pathologic characteristics in oral squamous cell carcinoma （OSCC）. Methods： TGF-β1 mRNA and protein epression in 48 cases of OSCC and 10 cases of normal controls were detected by in-situ hybridization and immunohistochemieal staining respectively, microves- sel density （MVD） in OSCC was measured. Statistical analysis was performed using t-test and chi-square test by SPSS 13.0. Results： TGF-β1 mRNA and protein were mainly detected in cytoplasm in OSCC cells. The expression of TGF-β1 mRNA and protein were significantly higher in OSCC than in normal oral tissues（ P 〈 0.01 ）. Lymph node metastasis and infiltration depth of OSCC cells were closely correlated with TGF-β1 expression（ P 〈 0.05 ） , but there was no significant correlation between the positive expression and sex, age of the patients and the differentiation degree of OSCC （P 〉 0.05 ）. TGFF-β1 expression was positively correlated with MVD in OSCC（P 〈0.01 ）. Conclusion： TGF-β1 overexpression in OSCC may play a potential role in the development, lymph node me- tastasis and the angiogenesis of OSCC.

关 键 词：口腔鳞状细胞癌 TGF-Β1 免疫组化 原位杂交 血管生成 

分 类 号：R739.8[医药卫生—肿瘤]