四株鸽Ⅰ型副黏病毒的分离鉴定及基因型分析  

Identification and genotypic analysis of four isolates of pigeon paramyxovirus type Ⅰ

作　　者：隋修锟[1] 李刚[1] 李铁 吴迪 程朝飞[1] 陶春爱[1] 黄华欣[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193 [2]合肥中大生物技术发展有限公司,安徽合肥230088

出　　处：《中国兽医科学》2013年第2期136-140,共5页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2008AA10Z411);国家自然科学基金项目(31172342);国家公益性行业科研专项(200903037);"十一五"国家科技支撑计划项目(2006BAD6A13-4)

摘　　要：为掌握安徽省鸽Ⅰ型副黏病毒病的流行特点及其与鸡新城疫各分离株的相互关系,对从安徽省不同鸽场鸽病料中分离的4株病毒(AH-2012、AH-2012-1、AH-2012-2、AH-2012-3),采用RT-PCR、血清学试验及基因序列测定等方法进行鉴定。结果显示,这些分离株均具有血凝性,并能使NDV标准阳性血清特异性抑制;PCR扩增出F基因特异性目的片段,其核苷酸序列与GenBank上登录的鸽Ⅰ型副黏病毒的同源性较高,相似性达94.8%;与传统的鸡源新城疫病毒标准株LaSota、F48E9的同源性较低,相似性约为80%;F基因编码蛋白的第112～117位氨基酸序列为R-R-Q-K-R-F。结果表明,这些毒株为鸽Ⅰ型副黏病毒,属于基因Ⅵ型。In order to study the epidemic characteristics of pigeon paramyxovirus typeⅠ（PPMV-Ⅰ） disease in Anhui Province and to demonstrate the relationship between pigeon paramyxovirus typeⅠ isolates and Newcastle disease virus（NDV） strains,4 isolates（AH-2012,AH-2012-1,AH-2012-2 and AH-2012-3）derived from pigeon farms were identified by RT-PCR,serological test and sequence analysis.As a result,the isolates exhibited hemagglutination and could be inhibited by the standard NDV positive serum.Specific fragment of partial F gene was obtained and cloned.Sequence alignment indicated that the F gene of the isolates shared close identity with NDV with similarity above 94.8%.However,when compared with classical strain LaSota and F48E9,the identity was lower with only 80% similarity.The amino acids from position 112-117 in F protein sequence was R-R-Q-K-R-F,which confirmed that the 4 isolates belong to genotypeⅥ of pigeon paramyxovirus.

关 键 词：鸽Ⅰ型副黏病毒(PPMV-Ⅰ) F基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]