一株甲醛降解菌的分离鉴定及降解条件研究  

Isolation,identification and degrading conditions of a formaldehyde-degrading bacterium

作　　者：钟卫鸿[1] 吴婉欣[1] 陈雯雯[1] 金晶[1] 邱乐泉[1] 钟莉[1] 吴石金[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2013年第1期25-30,共6页Journal of Zhejiang University of Technology

基　　金：国家863计划基金资助项目(2007AA061404)

摘　　要：从污水处理厂收集的土壤中分离到一株能降解甲醛的菌株,经过形态学分析、生理生化鉴定和16SrDNA序列比对分析,鉴定该菌为Methylobacteriumsp.XJLW.经驯化后,该菌株对甲醛的耐受由0.1g/L提高至1.2g/L.通过单因素实验得到该菌株降解甲醛的优化条件为:酵母膏1g/L,KH2PO40.7g/L,K2HPO40.8g/L,MgSO40.5g/L,温度30℃,pH 7.0.在优化后的条件下,培养52h后,该菌株对1.2g/L甲醛的降解率为31%.此外,该菌株的休止细胞8h后对2,15,30,45,60g/L的甲醛降解率分别为100%,96.8%,84.0%,26.5%,22.5%,具备较高的降解能力.A formaldehyde-degrading bacterium was isolated from soil of a wastewater treatment plant. Based on the physiological, biochemical test and 16S rDNA sequence analysis results the bacterium was identified as Methylobacterium sp. XJLW. After a period of domestication, the strain tolerance of formaldehyde concentration was up to 1.2 g/L. After single-factor tests, the optimal conditions were determined as follows： yeast extract 1 g/L, KH2PO40.7 g/L, K2HPO4 0.8 g/L, MgSO40.5 g/L, temperature 30 ℃, pH 7.0. Under the best conditions, 31% of 1.2 g/ L formaldehyde was degraded in 52 h. In addition, the formaldehyde degradation activity of the resting cells of Methylobacterium sp. XJLW was strongly high. When the initial concentration of formaldehyde was 2, 15, 30, 45, 60 g/ L, the formaldehyde degradation rates were 100%, 96.8%, 84%, 26.5%, 22.5% in 8 h, respectively.

关 键 词：甲醛 甲基杆菌 菌种鉴定 降解 休止细胞 

分 类 号：X172[环境科学与工程—环境科学]