高产琥珀酸菌株的等离子体诱变选育  

Screening of strain exposed under plasma discharge for improving succinic acid production

作　　者：汪晨[1] 蔡恒[1] 张恒丽[1] 张凯[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《南京工业大学学报（自然科学版）》2013年第1期57-60,共4页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家重点基础研究发展计划(973计划)(2011CB707405)

摘　　要：以谷氨酸棒杆菌作为原始出发菌株,通过常压室温等离子体诱变技术,确定高产琥珀酸等离子体诱变的物理参数和最佳诱变条件,采用在培养基中添加溴甲酚紫作为酸碱指示剂的方法,测定发酵液前后在波长590 nm处的吸光值,高通量筛选出在该波长处吸光值变化较大的突变株。结果表明:在诱变菌株的存活率为8%的条件下获得的突变株的正突变率达到22%。用40 g/L的葡萄糖对突变株进行摇瓶分批发酵,筛选得到诱变菌E7产琥珀酸15.6 g/L,乳酸22.8 g/L,该菌具备良好的产琥珀酸与有机酸的能力。The strain of Corynebacterium glutamicum was exposed on atmospheric and room temperature plasma （ARTP） jet to improve succinic acid production. The physical parameters of plasma discharge and the optimum condition of mutagenesis were established. Adding the bromocresol purple as a pH indicator to the medium, the broth absorbances were determined to obtain the mutants having large change in light absorption by high-throughput screening at the wavelength of 590 nm. Results showed that when the sur- vival rate of mutant strain was 8%, its positive mutation rate of Corynebacterium glutamicum cells was 22%. The results of the fed-batch culture in shake flask showed that the mutant strain E7 could produce succinic acid 15.6 g/L and lactic acid 22. 8 g/L from 40 g/L glucose. The mutant strain could produce succinic acid and total organic acid.

关 键 词：常压等离子体 琥珀酸 谷氨酸棒杆菌 诱变 

分 类 号：TQ921[轻工技术与工程—发酵工程]