HPLC法检测发酵液中赤藓糖醇含量  

Determination of erythritol in fermentation broth by HPLC

作　　者：孟小琴[1] 胡永红[1] 杨文革[1] 唐容容[1] 沈飞 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京210009 [2]南京新百药业有限公司,江苏南京210038

出　　处：《南京工业大学学报（自然科学版）》2013年第1期105-108,共4页Journal of Nanjing Tech University(Natural Science Edition)

基　　金：国家自然科学基金(31171644);江苏省科技支撑(农业)计划(BE2011354)

摘　　要：建立用高效液相色谱法(HPLC)测定发酵液中赤藓糖醇含量方法。采用Agilent NH2(4.6 mm×250 mm,5μm)色谱柱,以V(乙腈)∶V(水)=75∶25为流动相,柱温35℃,流速1.0 mL/min,检测器为示差折光检测器,进样量20μL。赤藓糖醇标准品质量浓度在0.2～10 mg/mL范围内线性关系良好,线性回归方程为Y=2.677 28X+0.218 88,相关系数r=0.999 23,相对标准偏差(RSD)=1.66%,试样回收率为98.1%～104.0%。实验结果表明该方法简便、快速、结果可靠。High performance liquid chromatography （HPLC） for the quantitative determination of erythritol in the fermentation broth was presented on a Agilent NH2 HPLC column（4.6 mm × 250 mm,5μm）. The mobile phase was acetonitrile-water of 75 ： 25 with the flow rate of 1.0 mL/min and the column temperature of 35 ~℃. The refractive index detector was used. The results showed that the external standard calibration curve for quantifying erythritol by HPLC was validated from 0. 2 mg/mL to 10 mg/mL with a satisfied linear relationship. The linear regression equation was Y = 2. 677 28X ＋ 0. 218 88, and the correlation coefficient was 0. 999 23. The recovery rate of the erythritol was 98.1%-104% with the relative standard deviation （RSD） of 1.66%. The experimental results showed that this method is simple, efficient and reliable.

关 键 词：赤藓糖醇 高效液相色谱法 发酵液 含量测定 

分 类 号：R917[医药卫生—药物分析学]